p,p'-DDD对人乳腺癌细胞的增殖作用

董力; 张明; 汤宁

p,p'-DDD对人乳腺癌细胞的增殖作用

基金项目: 中国疾病预防控制中心环境与健康相关产品安全所青年基金

详细信息

作者简介:
董力(1968-),女,硕士,副研究员;研究方向:分子生物及环境毒理学研究,E-mail:lizi1214@sina.com

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出版历程
- 刊出日期: 2017-06-25

Study of the Proliferation Effect of DDD on Mammary Cancer Cell MCF-7

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摘要

摘要:
[目的] 观察对,对-二氯二苯基二氯乙烷(p,p'-DDD)对人乳腺癌(MCF-7)细胞增殖的影响。

[方法] 采用体外培养 MCF-7细胞增殖试验检测 DDD的类雌激素活性, 并用生长曲线对其作用机制进行初步探讨。

[结果] DDD染毒剂量在 3& #215;10^-7 mol/L、3& #215;10^-6 mol/L时, 存在刺激 MCF-7细胞增殖的类雌激素活性(P < 0.05), 在 3& #215;10^-6 mol/L剂量时其增殖效应最大。细胞生长曲线方面, DDD组在第 3、5、7天各时间段细胞数均明显高于溶剂对照组(P < 0.05)。

[结论] DDD在 3& #215;10^-7 mol/L、3& #215;10^-6 mol/L具有刺激 MCF-7细胞增殖的类雌激素活性。
- 雌激素 /
- 对,对-二氯二苯基二氯乙烷 /
- 细胞增殖试验
Abstract:
[Objective] To study the proliferation effect of p,p'-DDD on human mammary cancer cell MCF-7.

[Methods] We detected estrogenic activity of DDD using the MCF-7 cell proliferation experiment in vitro, and analysed its mechanism by means of growth curve.

[Results] DDD in the dose of 3& #215;10^-7 mol/L and 3& #215;10^-6 mol/L could stimulate the MCF-7 cell proliferation and produce estrogenic activity, and the most proliferation effect dose was 3& #215;10^-6 mol/L. Cell cycle analysis of cell proliferation index indicated that DDD group was significantly higher than the control group at the third, fifth, and seventh day.

[Conclusion] DDD in the dose of 3& #215;10^-7 mol/L and 3& #215;10^-6 mol/L could stimulate the MCF-7 cell multiplication.
- estrogen /
- p,p'-DDD /
- cell proliferation