Objective To study the effects of carbon disulfide (CS2) on the expression of genes which are related with mitochondrion mediated sertoli-germ cells apoptosis in male rats.
Methods A 22-24 day-old SD male rat of clean grade was selected to establish the rat sertoli-germ cells co-culture system in vitro. According to the designed concentrations of CS2 (0, 1, 2, 4μmol/mL), the cells were divided into four groups accordingly and RNA were extracted. The mRNA expression levels of Cyto C, Apaf-1, Caspase-3, Caspase-9, Aif, EndoG, Smac, IAPS, Bax, Bcl-2 were detected by real-time fluorescent quantitative polymerase chain reaction (RT-PCR).
Results Comparing with the control group, the levels of Cyto C, Smac, IAPS, Caspase-3, Bax, and Bax/Bcl-2 mRNA in the sertoli-germ cells were increased, while the levels of Apaf-1, Aif, Bcl-2, and EndoG mRNA were decreased in CS2 exposure groups (P>0.05).
Conclusion CS2 exposure affects the expression of genes related with mitochondrial mediated apoptosis, and it probably relates to reproductive toxicity induced by CS2.
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