Objective To investigate the association between the heat shock protein 70 gene polymorphism and lung cancer.
Methods A case-control study was conducted among 159 lung cancer patients and 202 control subjects. Polymerase chain reaction-restrained fragment length polymorphism technique (PCR-RFLP) was applied to detect the single nucleotide polymorphisms (SNPs) of HSP70-1 +190 (G/C) and HSP70-2 +1267 (A/G). The interactions of the two gene polymorphisms and smoking and that of the two sites were analyzed by non-conditional Logistic regression.
Results There were significant differences between the case group and control group (χ2=19.16, P < 0.05; χ2=18.76, P < 0.05)in the distribution of each genotypes GG, GC and CC, and also in the frequencies of the two alleles G and C of HSP70-1 +190 (G/C). The CC genotype was moderately correlated with lung cancer. There were no significant differences in the distribution of genotypes and allele frequencies of HSP70-2 +1267 (A/G) between the case group and control group (P > 0.05). After stratification analysis according to smoking, it revealed that in heavysmokers subgroups who carried HSP70-1 +190 (G/C)GC or GC+CC genotypes and/or HSP70-2 +1267 (A/G)AA genotype were associated with significantly increased risk of lung cancer. The interaction analysis of genotype and smoking revealed that there was multiplication interaction between HSP70-1 +190 (G/C) polymorphism and smoking on susceptibility to lung cancer. There was also interaction between the two genotypes HSP70-1 +190 (G/C)and HSP70-2 +1267 (A/G)(χ2=12.87, P=.00).
Conclusion HSP70-1 +190 (G/C)polymorphism may be associated with the susceptibility to lung cancer in He'nan Han population, and there are multiplication interaction with HSP70-2 +1267 (A/G)polymorphism and smoking. The relationship between HSP70-2 +1267 (A/G) polymorphism and lung cancer has yet to be further studied and there may be an interaction between AA genotype and the heavy smokers.
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