Objective To examine the relationship between DNA damage and monoubiquitinated histone H2A in coke oven workers.
Methods A total of 106 coke oven workers(exposed group)and 73 water treatment workers(control group)were recruited in this study. The levels of urinary 1-hydroxypyrene(1-OHP)were determined using high performance liquid chromatography. Comet assay and Western blot were used to detect the DNA damage and the monoubiquitylation of histone H2A, respectively.
Results The mean Olive tail moments(OTM)(0.46)and urinary 1-OHP concentrations(0.34 μmol/mol Cr)in the exposed group were significantly higher than those in the control group(0.37 and 0.21 μmol/mol Cr, respectively, P<0.05). But the median of expression of monoubiquitinated histone H2A(0.61)in the exposed group were significantly lower than that in the control group(0.91, P<0.05). By taking OTM as the dependent variable, the results of multiple linear regression analysis showed that urinary 1-OHP and the relative expressions of monoubiquitinated histone H2A fit the equation as independent variables, and the coefficient of determination R2 was 0.383.
Conclusion DNA damage and urinary 1-OHP are closely related with the relative expression of monoubiquitinated histone H2A in the coke oven workers.
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