Objective To establish an ovarian tumor model in C57BL/6 mice with 7,12-dimethylbenzaanthracene (DMBA) injection and observe its biological features.
Methods Ten female mice were injected with DMBA under ovary membrane through microinjector in operation and under aenethsia.Necropsy after 4months observation,and then gross aspects,light microscopy,and other respects of the cancer bearing mice were examined.Preliminary observations on the biological characteristics of the tumor tissue were performed.
Results One mouse was died of severe infection after operation;two mice were tumor free,while the other seven were found bearing with undifferentiated carcinoma and/or aden carcinoma,respectively.Its biological features were quite comparable with ovarian tumor in woman.
Conclusion DMBA can induce ovarian tumor in C57BL/6 mice and it can serve as an animal model for study of ovarian tumor.
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