Objective To detect the toxic effects of tributyltin chloride (TBT) on cultured rat Sertoli cells and its injury mechanism.
Methods Sertoli cells were isolated from the testes of 20-23 day-old male SD rats and identified with hematoxylineosin staining (HE) and fas ligand immunohistochemical staining (FAS-L). To obtain more Sertoli cells, cultures were hypotonically treated with Tris-HCl. One blank control group, one solvent control group and four tributyltin chloride groups (20& #215;10-9, 40& #215;10-9, 80& #215;10-9, and 120& #215;10-9 mol/L, respectively) were set. Cell viability were determined by methyl thiazolyl tetrazolium (MTT), expression of proliferating cell nuclear antigen (PCNA) were determined by streptavidin-perosidase (SP), and the distribution of the cell cycles were analyzed by flow cytometry (FCM).
Results TBT can decrease the survival rate of Sertoli cells and interfere with the distribution of cell cycles. The result of immunocytochemistry showed that the expression of PCNA was significantly inhibited in the 80& #215;10-9 mol/L and 120& #215;10-9 mol/L dose groups.
Conclusion TBT can inhibit the proliferation and affect the cell distribution and PCNA expression of Sertoli cells.
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