Objective To study the effects of bisphenol A(BPA)on the morphology and β-HCG secretion of human early villus and the posible toxicity of BPA to human early villus
Methods The human early villus from forty preganancy cases(6-8 weeks)were collected, cultured and treated with BPA at different concentrations. The cultures were divided into 4 groups based on the concentration of BPA. The concentration of BPA was 1 000& #215;10-6 g/L in group A,500& #215;10-6 g/L in group B, 1& #215;10-6 g/L in group C respectively, and without BPA in group D as control. The concentration of β-HCG in the supernatant of culture was measured by chemiluminescence immunoassay at day 2 and day 4 of culture, and the cultured villus was stained by HE at day 4.
Results ① The concentration of β-HCG in the culture was elevated in A, B and C group with increasing culture time, while its concentration in D group was decreased. The concentration of β-HCG was statistically higher in group A, B and C than in group D. The concentration of β-HCG was increased with the increasing BPA.The differences of β-HCG values between day 4 and day 2 among the groups were statistically different(P=0.002).② The histologic abnormalities of villus were characterized by increasing syncytiotrophoblast knotting, prominent cytotrophoblast proliferating, basement membrane thickening, fibrinoid necrosis and anaplasia. In addition, atrophy was also observed in some villus.
Conclusion In culture, BPA is cytotoxic to villus and has effects on the secretion of trophocyte.
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