Objective To optimizing the solution concentration and procedures of pre-treatment in sputum analyses among patient with respiratory disease.
Methods Results of sputum induction with various solution and treatment in 40 patients with silicosis were compared.
Results Success result of sputum induction was obtained in rates of 80.00%, 82.50%, 60.00%, and 22.50% from inhalation of mist of 3%, 4%, 5%, and 6% hypertonic saline solution respectively. The differences between 4% and 5% to all others were statistically significant(P<0.05). Rate of successful result for 25 to 30 min. induction were 77.50% and 92.50%, respectively. Better result in dissolution and no influence level in detecting IL-6 and TNF-α were obtained from 30-50 min 37℃ water bath than less than 20 min. in 2-4 fold dilution of 0.1% DTT(P>0.05).
Conclusion The optimized condition for sputum induction was find of spraying mist of 3%-4% hypertonic saline for 20-30 min. Better consequent pre-treatment was 37℃ water bath incubation in 2-4 folds dilution with 0.1% DTT solution for 30-40 min.
DownLoad: