Objective To establish a high performance liquid chromatography-tandem triple quadrupole mass spectrometry method for simultaneous determination of 7 variants of microcystins in aquatic products.
Methods All samples were subject to extraction in Tris-HCl buffer (pH=7), clean-up with Oasis HLB solid phase extraction, C18 separation, mass spectrometry detection and quantification with internal standards.
Results After effective extraction and purification, 7 microcystins of LR, RR, YR, LA, LW, LY and LF were detected simultaneously. The mean recoveries spiked at levels of 10 μg/kg and 40 μg/kg in four matrices were 51%-112% and 60%-117%, with relative standard deviation of 1.7%-12.7% and 0.9%-8.0% (n=6), respectively. The limits of detection were 0.20-0.40 μg/kg.
Conclusion A rapid and practical method with high efficiency is proposed and provides technical basis for standard determination protocol of microcystins.
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