Objective To understand inflammation induced by the water-soluble and acid-soluble components of PM2.5 in rats having coronary atherosclerosis.
Methods Total of 48 Wistar rats were randomly divided into control group and coronary atherosclerotic model group (model group) (n=24). The control group was fed normal diet, and the model group was fed highcholesterol diet. 12 weeks later, coronary atherosclerosis in model group was confirmed pathological biopsy. The water-soluble components (WSC)and acid-soluble components (ASC)of PM2.5 were extracted. The control group and model group were further divided into 3 groups respectively. They were the normal control group, WSC control group, ASC control group, model control group, WSC model group, and ASC model group (n=8 each group). WSC control group and WSC model group (collectively called WSC group)were injected with WSC (40 mg/kg); ASC control group and ASC model group (collectively called ASC group)were injected with ASC (40 mg/kg); the normal control group and model control group (collectively called blank group)were injected with normal saline (NS). The rats were killed after 24 h of exposure. IL-6/TNF-α in serum and NF-κB in myocardial were measured.
Results The TNF-α in WSC group (2.83& #177;0.97)ng/mL was higher than the blank group (2.53& #177;0.76)ng/mL; the myocardial NF-κB activation level in WSC group (14.56& #177;10.58)% was higher than the blank group (7.33& #177;3.97)%. The myocardial NF-κB activation in the ASC group (18.80& #177;17.04)% was higher than the blank group (7.33& #177;3.97)%. Model of coronary atherosclerosis showed a synergistic effect on the WSC and ASC in increasing IL-6 and activating of myocardial NF-κB.
Conclusion WSC may increase TNF-α, and activate myocardial NF-κB; ASC may activate myocardial NF-κB. Coronary atherosclerosis model suggested an interaction among WSC and ASC in increasing IL-6 and activating NF-κB. The acid-soluble and water-soluble components of PM2.5 could contribute to the inflammation in coronary atherosclerosis in rats.
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