Objective To establish a novel method for lead determination in whole blood by graphite furnace atomic absorbance spectrometry (GFAAS).
Methods After the protein in whole blood was removed by 40 mL/L nitric acid mixed with 6 mL/L hydrogen peroxide in a centrifugal separator, lead in whole blood was determined by GFAAS with NH4H2PO4+Mg(NO3)2 as matrix modifier．
Results Of the current methodological study, the linear range was 10-400 μg/L, the detection limit was 4 μg/L, the recovery rate range was 87.8%-115.3%, and the relative standard deviation (RSD) was 3.6%-8.6%．
Conclusion Graphite furnace atomic absorbance spectrometry with nitric acid-hydrogen peroxide deproteinization is satisfied to the purpose of lead determination of whole blood.
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