Objective To identify and quantify musk xylene (MX) bioaccumulated in zebrafish (Danio rerio) and rare gudgeon (Gobiocypris rarus) by gas chromatography-mass spectrometry (GC-MS).
Methods The pretreatment of fish samples in cluded accelerated solvent extraction (ASE) followed by gel permeation chromatography (GPC). The eluate was determined by GC-MS after concentrated. MX standard solution was used as external standard.
Results The detection limit of this method was 4 μg/L; the range of linearity was between 10 μg/L and 400 μg/L, presenting a good linear relationship. Using this method, the recovery rates of 20 μg/L and 100 μg/L standard MX solution added to the zebrafish samples were 108.0% and 97.4% respectively; the relative standard deviations were 4.9% and 9.3% respectively. The recovery rates of 20μg/L and 100μg/L standard MX solution added to the rare gudgeon samples were 104.0% and 112.0% respectively; the relative standard deviations were 10.0% and 10.0% respectively. Both results met the requirements of recovery rate (80%-120%) and relative deviation (<15%).
Conclusion This method is featured by high sensitivity, wide linear range, and low impurity interference. The results show that the method is suitable for detection of MX in rare gudgeon.
DownLoad: