Objective To optimize and evaluate the effectiveness of detection methods for Salmonella in preventive occupational physical examination.
Methods Using the stock of Salmonella strains we tested the susceptibility and specificity among CHROM agarTM Salmonella (CAS), Xylose Lysine Deoxycholate agar (XLD)and Wuhan Salmonella-Shigella agar (WS). Using single blind method, we detected 1 717 anal swabs from food-handlers during their occupational physical examination to compare the effectiveness of isolating Salmonella in CAS and WS after selenite F broth (SF)enrichment, and also detected 1 000 anal swabs of them to compare the effectiveness of isolating Salmonella in CAS and WS after selenite brilliant green enrichment broth(SBG) and SF, respectively. Finally, we evaluated the application of isolating Salmonella by optimized process of SBGXLD/CAS from anal swabs in the course of preventive occupational physical examination in Jing'an District.
Results The susceptibility and specificity of CAS and XLD from the stock Salmonella strains and on site detection were significantly higher than that of WS. The results from optimum method of SBG-XLD combining process were significantly superior to that of the SF-WS. In Jing'an District, the positive rate of Salmonella in occupational physical examination in 2008 increased by eight times than in 2006 and ten times than in 2007. Monthly positive rate of Salmonella increased by 2-10 times than before. The peak value of positive rate was over 1% in July and Augest.
Conclusion CAS may be applied to isolate all Salmonellae including Salmonella typhi and Salmonella paratyphi. The SBG-XLD/CAS combining process has high susceptibility and easy to grasp. This optimum method is suitable for routine isolating Salmonella in preventive occupational physical examination.
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