Objective To explore the relationship between chromosomal damage induced by 1,3-butadiene(BD) and polymorphisms of metabolizing enzymes of CYP2E1 and GSTs.
Methods Cytokinesis-block micronucleus(CBMN) assay was used to detect chromosomal damage in peripheral lymphocytes of 166 1,3-butadiene exposed workers and 41 controls. PCRRFLP technique was applied to detect polymorphisms in CYP2E1 c1c2. Null genotypes of GSTT1 and GSTM1 were detected by PCR.
Results Compared the CBMN frequencies of the two groups we found that the frequency in exposed workers was higher than that in control group (P<0.01). Individuals with the CYP2E1 c1c2/c2c2 and GSTM1 positive genotype were observed to have significantly higher CBMN frequencies than individuals with more common genotypes (χ2=14.39, P<0.01 and χ2=4.23, P<0.05). Sex, age, duration of work, smoking and drinking habit had no relationship with CBMN frequencies.
Conclusion BD-exposed workers had higher risk of chromosomal damage compared with control. Cytokinesis-block micronucleus(CBMN) assay can be used to evaluate the early damage of butadiene-exposed workers. CYP2E1 c1c2/c2c2 and GSTM1 positive genotype showed higher average CBMN frequencies than those with more common genotypes.
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