Objective To investigate the acute effects of cadmium on primary rat gonocyte cell/sertoli co-cultures.
Methods Gonocytes and sertoli cells were separated from 3-day SD rat. Cadmium (0, 2.5, 5, 10, 20 μmol/L)were added to coculture system 12 h after gonocyte and sertoli cell had co-cultured for 24 h. Alkaline phosphatase and oilred O were used to identify the gonocyte and sertoli cells. TUNEL in situ detection was used for detecting apoptosis.
Results Exposure of 2.5 μmol/L cadmium did not induce morphologic change of gonocytes and sertoli cells. When exposure dose greater than 5 μmol/L, sertoli cells showed morphological change identified by oilred O in a dose-dependent manner. TUNEL in situ detection suggested that cadmium in creased apoptosis in both gonocytes and sertoli cells.
Conclusion Exposure of cadmium for 12 h induced morphological change of sertoli cells and apoptosis of both gonocyte and sertoli cell.
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