Objective To develop a simplified pretreatment method for simultaneous concentration determination of seven hormones in cosmetics via high performance liquid chromatography (HPLC).
Methods After a simplified pretreatment of the samples, the proper HPLC conditions were optimized.
Results This simplified pretreatment was good enough for the measurement. An XTerra TMRP18 column was employed and a mixture of acetonitrile:aqueous (40:60)was used as mobile phase. The measuring wavelength was 230 nm. The proper HLPC conditions were investigated for the determination of seven hormones in the linear range 0.1-50.0 mg/L (correlation coefficients greater than 0.999, and the recoveries for the seven sex hormones ranged from 84%-104% with relative standard deviations of 1.9%-8.3%. The lowest detection limits were 0.01-0.06 mg/L.
Conclusion The method is suitable for sitmultaneous determination of the seven sex hormones in cosmetics with simplicity and accuracy.
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