Objective To establish an analytical method for the determination of trace content of microcystins (MC-RR, MC-YR and MC-LR)and nodularin (NOD)in water sample.
Methods After cleaned and enriched by solid phase extraction (SPE)using C18, the water sample was determined by ultra performance liquid chromatography/tandem mass spectrometry (UPLCMS/MS)in multi-reaction-monitoring mode. Matrix-matched standard curves were used to compensate the matrix effect.
Results The recoveries were in range of 95.7%-115.0% with relative standard deviation(RSD)2.43%-6.04%. The detection limits were 0.05-0.20 ng/L.
Conclusion An analytical method for the determination trace microcystins and nodularin in water sample was established by UPLC-MS/MS. This method could be used to analyze local water samples.
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