Objective To explore the associations of polymorphisms in interleukin-1A (IL-1A) and IL-1B genes with coronary heart disease (CHD).
Methods CHD cases were diagnosed according to WHO criteria at three of the major hospitals in Wuhan. A total of 1 003 patients with confirmed CHD and 1 003 age-and sex-matched controls were included in the present study. Participants' information and data were obtained by a standard questionnaire. Polymorphisms of IL-1A and IL-1B genes were detected by Taqman single nucleotide polymorphism (SNP)allelic discrimination by means of an ABI 7900HT.
Results It indicated that SNP rs17561(ala114ser) in exon of IL-1A gene was associated with higher risk of CHD. Compared with GG genotype, subjects with TT had more than 3-fold risk of CHD. Four SNPs in IL-1B gene were not significantly associated with CHD. Further haplotype analysis did not find any significant association with CHD.
Conclusion These results suggested that SNP rs17561 in IL-1A gene was associated with higher risk of CHD. This finding needed to be validated in further studies.
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