Author Submission Open Access
Advance Search
Volume 28 Issue 4
Jul.  2017
Turn off MathJax
Article Contents
Abstract
Related Articles
WANG Xiao-Yan , LI Na , NIU Qiao , NIE Ji-Sheng . The Dose-effect and Time-effect Expression of Histone Deacetylase 2 mRNA in B(a)P-induced Neural Cell Apoptosis[J]. Journal of Environmental and Occupational Medicine, 2011, 28(4): 219-223.
Citation: WANG Xiao-Yan , LI Na , NIU Qiao , NIE Ji-Sheng . The Dose-effect and Time-effect Expression of Histone Deacetylase 2 mRNA in B(a)P-induced Neural Cell Apoptosis[J]. Journal of Environmental and Occupational Medicine, 2011, 28(4): 219-223.
shu

The Dose-effect and Time-effect Expression of Histone Deacetylase 2 mRNA in B(a)P-induced Neural Cell Apoptosis

More Information
  • Corresponding author:

    NIU Qiao ,Email:niuqiao55@163.com

    NIE Ji-Sheng ,Email:niejisheng@126.com

  • Received Date: August 02, 2010
    Graphical Abstract
    • Abstract
  • [Objective] To investigate the dose-effect and time-effect expression of histone deacetylase 2(HDAC2) mRNA in B(a)P-induced neural cell apoptosis in vitro.

    [Methods] The primary cultured neurons of rat were assigned into two lots. The first lot was separated into 4 groups and treated with B(a)P at final concentrations as 0 μmol/L(DMSO group), 10 μmol/L (low-dose group), 20 μmol/L(medium-dose group), and 40 μmol/L(high-dose group)respectively, and added S9 at the same time, then incubated for 48 hours. The second lot was separated into 5 groups, all treated with 20 μmol/L B(a)P, then incubated for 0, 6, 12, 24, 48 h respectively. The apoptosis rate of all incubated neurons was detected by flow cytometry, the expression of caspase-3 and HDAC2 mRNA by QRT-PCR.

    [Results] ① Flow cytometry showed that compared with the DMSO group, the apoptosis rates of neurons in middle and high-dose group were significantly increased(P<0.05); compared with the low-dose group, the apoptosis rates also increased(P<0.05). The apoptosis rates at 24, 48 h after exposure were significantly increased compared with that at 0 h(P<0.05). ② The caspase-3 and HDAC2 mRNA expression showed significant increments in highdose group compared with DMSO group(P<0.01). The caspase-3 mRNA expression in high dose group increased significantly compared with the low-dose group(P<0.05). The expressions of caspase-3 and HDAC2 mRNA at 48 h after exposure increased significantly compared with that at 0 h(P<0.01, P<0.05).

    [Conclusion] After exposed to B(a)P, neural cell apoptosis and HDAC2 mRNA expression increased. HDAC2 may play an important role in B(a)P-induced neuronal apoptosis.

    • FullText(HTML)
    • References (0)
    • Related Articles

  • Related Articles

    [1]WANG Yong, CHEN Xiaoman, LIU Mengya, LUO Hao, WANG Yongli, LIU Xiaoming, QIN Xiujun. Effect of hydrogen-rich water on brain injury in rats with acute radiation based on PI3K/AKT/Caspase-9 signaling pathway[J]. Journal of Environmental and Occupational Medicine, 2023, 40(3): 246-253. DOI: 10.11836/JEOM22406
    [2]LI Li-ping, WANG Le, DE Xiao-ming, LI Ling, ZHANG Ya-juan, ZHU Li-min. Effects of bisphenol A on apoptosis and insulin secretion of insulinoma cells in rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(6): 609-615. DOI: 10.13213/j.cnki.jeom.2020.19793
    [3]CAO Hong-ting, SUN Zu-yue, ZHOU Li, LUO Yu-zhen, ZHOU Jian, WANG Ling, CHEN Jian-mao, LI Xiao-yu, SONG Qi-ru. Effects of lindane on cleaved caspase-3 expression in spermatogenic cells and serum luteinizing hormone and testosterone concentrations in rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(5): 508-513. DOI: 10.13213/j.cnki.jeom.2020.19869
    [4]GAO Yan-rong, SHI Xue-min, JIA Yu-qiao, WANG Li, TAI Dai-peng, SUN Yao, CHANG Xiang-yu, ZHANG Han, TIAN Jiang, LIU Lu-min, LI Dong-ying. Effects of organic extracts in source water in Baotou section of the Yellow River on germ cell apoptosis and 3-NT/Caspase-3 expressions in testis of male mice[J]. Journal of Environmental and Occupational Medicine, 2019, 36(12): 1137-1142. DOI: 10.13213/j.cnki.jeom.2019.19272
    [5]WANG Nan-lan, LEI Lu, AO Quan, CHEN Mi, WU Yan-qiu, ZHANG Hua, WEI Yan. Effect of sodium fluoride on apoptosis of mice osteoblasts MC3T3-E1 cells[J]. Journal of Environmental and Occupational Medicine, 2017, 34(4): 330-336. DOI: 10.13213/j.cnki.jeom.2017.16756
    [6]WANG Wu-bin, ZHANG Hong-jie, LIU Yan-li, YANG Jin. Effects of ubiquitin protein ligase RING2 on BPDE-DNA adducts and CHK1 in BEAS-2B cells exposed to benzo (a) pyrene[J]. Journal of Environmental and Occupational Medicine, 2017, 34(3): 189-195. DOI: 10.13213/j.cnki.jeom.2017.16616
    [7]LIU Xiao-hui , HUANG Dan , WANG Ruo-xi , SHAO Jing , YAN Jin-song . Possible Involvement of Lysosomes in 2, 2', 4, 4'-Tetrabromodiphenyl Ether Mediated Apoptosis in HepG2 Cells[J]. Journal of Environmental and Occupational Medicine, 2013, 30(7): 510-514.
    [8]LI Cui-zhen , HUO Qian , ZHANG Chen , ZHOU Zhi-jun , WU Qing . Effects of Benzo(a)pyrene on mRNA Expression of Junction Protein Genes in Primary Cultured Sertoli Cells[J]. Journal of Environmental and Occupational Medicine, 2013, 30(3): 161-166.
    [9]CHEN Xi , WANG Jian-shu , JIANG Ying , YANG Guang-tao , WANG Jing , WANG Qian , RAO Kai-min , YUAN Jing . Effects of Co-exposure of Di-(2-ethylhexyl) Phthalate and Benzo(a)pyrene on CYP1A1 and CYP3A4 Enzyme Activities in HepG2 Cells[J]. Journal of Environmental and Occupational Medicine, 2011, 28(9): 525-530.
    [10]LI Na , WANG Xiao-yan , LIU Chun-chang , ZHANG Qin-li , NIU Qiao . Effect of caspase-3 Gene on Aluminum Induced Neuronal Apoptosis in Mice[J]. Journal of Environmental and Occupational Medicine, 2011, 28(3): 137-140.

Catalog

    Get Citation
    PDF
    XML
    Article views (2573) PDF downloads (676) Cited by()

    Address：A440, 1399 Shenhong Road, Shanghai, ChinaPostal Code：201107Tel（Fax）：021-62084529Email：zazhi2@scdc.sh.cn

    京ICP备17028258号-5

  • wechat

    • WeChat

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return