Objective To establish a method for rapid determination of thiocyanate in urine by ion chromatography.
Methods The urine samples were diluted by water, then centrifugated and filtered through 0.22 μm membrane, following by online purification with IonPac NG1 pre column. The purified samples were separated through an IonPac AS16 anion column and eluted by KOH with a flow rate at 1.0 mL/min. Thiocyanate was detected by conductivity detector, and quantification was determined by external standard method. The experimental conditions were optimized, and the parameters of the proposed method including detection limit, stability, accuracy, and precision were calculated.
Results A linear relationship was found between the values of peak area and the mass concentrations of thiocyanate in the range of 0.10-25.0 mg/L. The correlation coefficient was 0.999 8, the limit of detection (LOD) was 0.04 mg/L, and the limit of quantification (LOQ) was 0.10 mg/L. The recovery rates were in the range of 96.6%-105.1% at three spiked concentrations of 0.5, 5.0, and 20 mg/L, with relative standard deviations (n=6) ranging from 1.3% to 2.6%.
Conclusion This simple, rapid, and accurate method is suitable for determination of thiocyanate in human urine.
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