Objective To explore the relationship between DNA repair capacity (DRC) and chromosomal damage induced by 1, 3-butadiene (BD).
Methods Personal information including occupational history, age, gender, smoking, and drinking status was collected using questionnaires. Gas chromatography was used to detect the BD level in the selected working environment. DRC in peripheral blood lymphocytes among 60 BD-exposed workers and 60 non-exposed controls was measured by the repair rate of bleomycin-induced DNA damage using chromatid breakage assay.
Results The average concentration of BD in the working environment was 1.80 (0.59-2.76) mg/m3. The chromatid break rates (break event per cell, b/c) were higher in the BD-exposed workers than those in the controls (1.06& #177;0.41)% vs. (0.85& #177;0.36)%, P<0.01. The b/c of alcohol drinkers in the BD-exposed workers was higher than that of non-drinkers.
Conclusion Decreased DRC may be an important biological event in BD carcinogenesis.
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