Objective To probe the DNA damage and activation of Epstein-Barr virus early antigen (EBV-EA) induced by 900 MHz mobile phone radiofrequency radiation at an maximum power density of 10.644 µW/cm.
Methods NIH/3T3 cells were exposed to mobile phone radiation for 0,1,2,4,6,8,12h,respectively and followed by DNA damage detection via comet assay;corresponding control groups were also set.Raji cells were divided into four groups:A,radiation (-) TPA (12-0-tetradecanoylphorbol-13-acetate)(-);B,radiation (-) TPA (+);C,radiation (+) TPA (-);D,radiation (+) TPA (+).Group C and D were exposed to mobile phone radiation,four hours per day,for four weeks.At the end of each week,group B and D were treated with 1 ng/mL TPA for forty-eight hours.Immunocytochemistry method was used to detect the activation of EBV-EA.
Results The Olive tail moment of NIH/3T3 cells were significantly increased after exposed to the designed mobile phone radiation over two hours (P < 0.05).However,there were no significant increases in the other time points (P > 0.05).Moreover,after being exposed for four weeks,the positive rates of EBV-EA in group C and D were higher than that in group A (P < 0.05),and especially in the TPA treated group D showing the highest positive rate (P < 0.01).
Conclusion Mobile phone radiofrequency radiation could cause damage to cellular DNA with the designed intensity and exposure protocol,and may activate the expression of EBV-EA.
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