Abstract:
[Objective] To explore the effect of haze particulate matter PM2.5 on apoptosis of human bronchial epithelial cells (16-HBE).
[Methods] Groups of cultured 16-HBE cells were exposed to 8, 16, 32, 64 and 128μg/mL PM2.5 respectively for 24, 48 and 72 h of incubation to detect the cell survival and apoptosis rates.
[Results] After 24, 48 and 72 h of interference, compared with the negative control group, the cell survival rates of 64 and 128μg/mL groups decreased significantly (P < 0.05), and the cell total apoptosis rates increased significantly (P < 0.05). Compared with the corresponding 24 h groups, the cell survival rates of 72 h groups decreased significantly (P < 0.05), and the apoptosis rates increased significantly except when treated with 8 μg/mL (P < 0.05).
[Conclusion] Haze particulate matter PM2.5 possess cytotoxic effect on 16-HBE to induce apoptosis in a dose-and time-dependent manner.
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