天门冬水煎剂对氟染毒大鼠脑组织SIRT1和BDNF表达的影响
Effects of asparagus decoction on expressions of SIRT1 and BDNF in brain tissues of rats exposed to fluoride
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摘要:
目的 探讨天门冬水煎剂对氟染毒大鼠脑组织沉默信息调节因子2相关酶1（SIRT1）和脑源性神经营养因子（BDNF）表达的影响。
方法 将清洁级SD大鼠60只随机分为对照组（自由食用正常玉米饲料）、染氟组（食用50 mg/kg含氟饲料）、天门冬低剂量治疗组（2.5 g/kg天门冬+50 mg/kg含氟饲料）、天门冬中剂量治疗组（5 g/kg天门冬+50 mg/kg含氟饲料）、天门冬高剂量治疗组（10 g/kg天门冬+50 mg/kg含氟饲料），每组12只，雌雄各半。以自由食用含氟饲料方式进行染毒，并以灌胃方式给药天门冬水煎剂，每天1次，1 mL/次，连续3个月，采用实时定量荧光聚合酶链反应（qRT-PCR）和免疫印迹法检测脑组织SIRT1、BDNF的mRNA和蛋白表达水平。
结果 染氟组和不同剂量天门冬组大鼠脑组织SITR1、BDNF的mRNA和蛋白水平均低于对照组，且差异均有统计学意义（均P＜0.05）。天门冬中剂量和高剂量组大鼠脑组织SITR1、BDNF的mRNA和蛋白表达水平均高于染氟组，差异有统计学意义（P＜0.05）。各天门冬剂量组比较，随天门冬剂量增加，大鼠脑组织SITR1、BDNF的mRNA和蛋白表达水平逐渐升高（P＜0.05）。光镜下观察染氟组大鼠脑组织星形细胞明显减少，细胞出现肿胀，核仁消失，呈现明显损伤的现象；各天门冬组大鼠脑组织星形细胞逐渐增加，核仁逐渐清晰，较染氟组明显改善。
结论 在本实验剂量下，天门冬表现出一定的脑保护作用，可能与天门冬上调氟染毒大鼠脑组织SITR1、BDNF的mRNA和蛋白水平有关。
Abstract:Objective To investigate the effects of asparagus decoction on the expressions of silent mating type information regulation 2 homolog 1 (SIRT1) and brain-derived neurotrophic factor (BDNF) in brain tissues of rats exposed to fluoride.
Methods Sixty clean SD rats were randomly divided into control group (corn feed), fluoride group (fluoride 50mg/kg), asparagus low-dose treatment group (asparagus 2.5 g/kg + fluoride 50 mg/kg), asparagus moderate-dose treatment group (asparagus 5 g/kg + fluoride 50 mg/kg), and asparagus high-dose treatment group (asparagus 10 g/kg + fluoride 50 mg/kg), with 12 rats in each group, half male and half female. The rats were exposed to fluoride by free feeding and administered by gavage with asparagus decoction, once a day, 1 mL/time, for three continuously months. Brain SIRT1 and BDNF mRNA and protein levels were detected by real-time quantitative PCR and Western blot.
Results The levels of SITR1 and BDNF mRNA and protein in brains of rats exposed to fluoride and different doses of asparagus were lower than those in the control group (Ps < 0.05). The levels of SITR1 and BDNF mRNA and protein in brains of the moderateand high-dose asparagus treatment groups were lower than those in the fluoride group (Ps < 0.05). The levels of SITR1 and BDNF mRNA and protein in rat brains increased gradually with increasing asparagus dose (P < 0.05). Under optical microscopy, reduced astrocytes in the brain tissues of rats exposed to fluoride were observed, as well as swollen cells and disappearing nucleolus, showing obvious damage; increasing astrocytes in the brain tissues of rats treated with asparagus were observed, as well as clear nucleolus compared with the fluoride group.
Conclusion Asparagus at designed doses has a protective effect on brain tissues post floride exposure, and the mechanism may be related to the up-regulation of mRNA and protein levels of SITR1 and BDNF in brain tissues of rats exposed to fluoride.
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