Abstract:
Objective To establish a method for determination of four arsenic species in drinking water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS).
Methods Arsenic speciation in water was separated by high performance liquid chromatography, and qualitatively and quantitatively determined by atomic fluorescence spectrometry. Experimental conditions were optimized including potassium borohydride mass fraction, hydrochloric acid volume fraction, sodium hydroxide mass fraction, buffer concentration, and solution pH. Then the method was applied to actual water samples.
Results The detection limits of arsenic (Ⅲ), monomethylarsenic acid (MMA), dimethylarsenic acid (DMA), and arsenic (Ⅴ) were 0.36, 0.69, 0.39, and 2.34 μg/L, respectively. All correlation coefficients were greater than 0.999. The standard recovery rates were 95.7%-107.9%. The relative standard deviations were 0.23%-9.31%.
Conclusion HPLC-HG-AFS is featured by low detection limit and high precision with accurate and reliable testing results for determination of arsenic speciation in drinking water.
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