苯暴露对小鼠血液系统和DNA氧化损伤的影响

Effects of Benzene on Blood System and DNA Oxidative Damage in Mice

  • 摘要:
    目的 观察苯吸入染毒对小鼠的周围血象、尿中苯巯基尿酸(SPMA)和8-羟基脱氧鸟苷(8-OHdG)的影响,探讨苯暴露对血液系统和DNA氧化损伤的作用。
    方法 将120只昆明种雄性小鼠随机分为4组,每组30只,低、中、高组苯染毒质量浓度分别为375、750和1 500 mg/m3,对照组吸入空气。采用静式吸入染毒法,每天2 h,每周5 d,持续染毒3个月。分别于染毒后1、2、3个月时,每组随机处死10只小鼠,检测周围血象、尿中SPMA和8-OHdG的水平。
    结果 白细胞、红细胞、淋巴细胞计数均随着染毒浓度的升高而降低(P < 0.05);尿中SPMA及8-OHdG水平随着苯染毒浓度的增加而升高(P < 0.05)。高浓度组染毒3个月时尿中8-OHdG水平明显高于1、2个月(P < 0.05)。尿中SPMA及8-OHdG与白细胞计数均呈负相关(rSPMA=-0.718,r8-OHdG=-0.971,均P < 0.01)。
    结论 亚慢性苯暴露可导致小鼠血液系统和DNA氧化损伤。

     

    Abstract:
    Objective To observe the changes of peripheral hemogram, urinary S-phenylmercapturic acid (SPMA), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in mice as indicators of blood system and DNA oxidative damage after benzene inhalation.
    Methods A total of 120 Kunming male mice were exposed to 0, 375, 750, and 1 500 mg/m3 of benzene for 2 h each day, 5 days each week, in static inhalation cabin for three months. In each group ten mice were randomly sacrificed every month. Hemogram and urinary SPMA and 8-OHdG were measured.
    Results With higher benzene exposure dose, the counts of white blood cells (WBC), red blood cells (RBC), and lymphocytes were reduced (P < 0.05), but the levels of SPMA and 8-OHdG in urine were increased (P < 0.05). Higher urinary 8-OHdG level was found in the mice with three months of 1 500 mg/m3 benzene inhalation than those with one or two months of exposure (P < 0.05). Urinary SPMA and 8-OHdG were negatively correlated with WBC (rSPMA=-0.718, r8-OHdG=-0.971, P < 0.01).
    Conclusion Subchronic exposure to benzene might induce blood system and DNA oxidative damage in mice.

     

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