Serum concentrations of perfluoroalkyl and polyfuoroalkyl substances and determinants in men of childbearing age
M（ P25， P75）分别为8.31（4.90，17.79）ng·mL−1和2.77（2.18，3.46）ng·mL−1。其中6种PFASs（PFOA、PFNA、PFDA、PFUdA、PFHxS和PFOS）检出率为100%，其次为PFDoA和PFTrDA，检出率分别为87.61%和88.59%。多因素线性回归结果显示：年龄与Σ8PFASs（检出率大于80%的8种PFASs浓度之和）呈正相关（ P<0.05）；家庭月总收入2000~<4000元的男性比家庭月总收入<2000元的男性血清PFOA浓度高53.73%（ P<0.01）；家庭月总收入≥4000元的男性比家庭月总收入<2000元的男性血清PFNA和PFTrDA的浓度均高32.31%（ P<0.05）；生活中使用塑料茶杯的男性比生活中不使用经口接触塑料制品（塑料餐具、塑料茶杯和塑料袋打包食物）的男性血清PFHxS浓度高33.64%（ P<0.01）；使用肥皂洗澡的男性比不使用清洗剂洗澡的男性血清PFHxS、PFOS和Σ8PFASs浓度分别高33.64%（ P<0.01）、43.33%（ P<0.01）和36.34%（ P<0.05）；使用香皂洗澡的男性比不使用清洗剂洗澡的男性血清PFHxS浓度高34.99%（ P<0.05）。 结论
Perfluoroalkyl and polyfluoroalkyl substances (PFASs) exposure may affect male reproductive health. There are regional differences in PFASs exposure levels among men of childbearing age in China, and current research results on associated influencing factors are inconsistent.
To investigate the levels of PFASs in serum and their determinants among men of childbearing age.
The participants (
n=113, 22-45 years old) were from a cross-sectional study of exposure to environmental pollutants and male reproductive health damage in Hubei Province conducted from April to June 2013 at the Reproductive Medicine Center of Tongji Hospital, Wuhan, Hubei Province. Eleven kinds of PFASs were measured in serum by isotopic dilution-high performance liquid chromatography-tandem mass spectrometry. The included PFASs were prefluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUdA), perfluorododecanoic acid (PFDoA), perfluorotridecanoic acid (PFTrDA), perfluorotetradecanoic acid (PFTeDA), perfluorohexane sulfonate (PFHxS), and perfluorooctane sulfonate (PFOS). Information about participants' demographic characteristics, lifestyle, and habits was collected by a set of self-designed questionnaires. The associations of demographic characteristics, lifestyle, and habits with exposure to PFASs were analyzed by linear regression. Results
The major components of PFASs were PFOS and PFOA, and the concentrations expressed as
M( P25, P75) were 8.31 (4.90, 17.79) ng·mL−1 and 2.77 (2.18, 3.46) ng·mL−1, respectively. The positive rates of six PFASs (PFOA, PFNA, PFDA, PFUdA, PFHxS, and PFOS) were 100%, followed by PFDoA and PFTrDA (87.61% and 88.59%, respectively). The linear regression results showed that age was positively associated with the levels of Σ8PFASs (sum of the concentrations of the eight PFASs with a positive rate greater than 80%) ( P< 0.05). The concentration of serum PFOA in men with monthly family income of 2000-4000 yuan was 53.73% ( P< 0.01) higher than those in men with monthly family income of <2000 yuan. The serum concentrations of PFNA and PFTrDA were both 32.31% ( P< 0.05) higher in men with monthly family income ≥4000 yuan than those in men with monthly family income <2000 yuan. The serum concentration of PFHxS in men who used plastic cups was 33.64% ( P< 0.01) higher than that in men who did not report oral contact with plastic products (plastic tableware, plastic cups, and plastic bags for packing food). The serum concentrations of PFHxS, PFOS, and Σ8PFASs were 33.64% ( P< 0.01), 43.33% ( P< 0.01), and 36.34% ( P< 0.05) higher in men who bathed with laundry soap than those in men who did not use detergents. Men who bathed with toilet soap had a 34.99% ( P< 0.05) higher serum concentration of PFHxS than those who bathed without detergents. Conclusion
Men of childbearing age are exposed to PFASs extensively. Age, monthly household income, usage of laundry soap or toilet soap in bathing, and usage of plastic cups may influence the level of PFASs in serum. However, further investigation is needed to confirm these results.