Abstract:
Objective To observe the effect of sucrose on pathological and ultrastructural change of pancreas in GotoKakizaki (GK)and Wistar rats.
Methods GK (n=26) and Wistar rats (n=26)were randomly divided into four groups:Wistar(W)group, Wistar+30% sucrose(WS)group, GK(G)group, GK+30% sucrose(GS)group. WS and GS group were fed with 30% sucrose water, while W and G group with plain water. All animals were sacrificed after 6 weeks. The pathological changes of pancreas were observed using histochemical staining and electro-microscope.
Results After fed with 30% sucrose water, fibrosis of islets was seen and the number of pancreatic β-cell decreased significantly in WS group. Furthermore, irregular deform of nucleus, significant reduction of the secretary granules within cells, and vacuolization of mitochondria was demonstrated in WS group. Compared with WS group, more serious fibrosis of islets was observed and pancreatic β-cells were only sporadically visible in GS group; in addition, kayro-pyknosis, substantial reduction of the secretary granules in cells, severe vacuolization of mitochondria, ridge dissolution and fracture, and severe pancreatic pathological changes were also observed in GS group.
Conclusion High sucrose intake could induce and aggravate pancreatic pathological lesions in GK and Wistar rats, suggesting that GK rats fed with 30% sucrose water could be used as an animal model for non-obese diabetes study.
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