甲基叔丁基醚对加油站职业人群血清淋巴细胞DNA的损伤

胡大林; 彭晓武; 彭晓春; 唐焕文; 袁建辉; 刘云岗; 卫秦芝; 张文娟; 杨建平; 沙焱; 方道奎; 庄志雄

甲基叔丁基醚对加油站职业人群血清淋巴细胞DNA的损伤

1.
南方医科大学公共卫生与热带医学学院毒理学系, 广东 510515
2. 环保部华南环境科学研究所环境与健康室, 广东 510655
3. 广东医学院公共卫生学院环境与健康研究所, 广东 523808
4. 深圳市疾病预防控制中心毒理室, 广东 518055
5. 深圳市宝安区疾病预防控制中心职业卫生科, 广东 518101
6. 深圳市职业病防治院职业卫生科, 广东 518001
7. 中山大学公共卫生学院预防医学系, 广东 510080

基金项目: 国家环保部公益性行业科研专项基金项目(编号:201009008)

详细信息

作者简介:
胡大林(1971-),男,医学博士,教授;研究方向:环境毒理学;E-mail:smuhdl@126.com

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出版历程
- 收稿日期: 2013-04-26
- 刊出日期: 2017-06-23

Lymphocyte DNA Damage Induced by Methyl Tert-Butyl Ether in Gas Station Attendants

1.
Department of Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, Guangdong 510515, China
2. Department of Environment and Health, South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangdong 510655, China
3. Environment and Health Institute, School of Public Health, Guangdong Medical College, Guangdong 523808, China
4. Department of Toxicology, Shenzhen Center for Disease Control and Prevention, Guangdong 518055, China
5. Department of Occupational Health, Baoan District Center for Disease Control and Prevention of Shenzhen, Guangdong 518101, China
6. Department of Occupational Health, Shenzhen Prevention and Treatment Center for Occupational Diseases, Guangdong 518001, China
7. Department of Preventive Medicine, School of Public Health, Sun Yat-sen University, Guangdong 510080, China

摘要

摘要:
[目的] 探索汽油添加剂甲基叔丁基醚(methyl tert-butyl ether,MTBE)对加油站职业暴露人群DNA的损伤。

[方法] 选择华南地区8 家加油站工作人员100 名为调查对象(其中暴露组61 人,对照组39 人),进行健康检查和问卷调查;调查对象各抽取外周静脉血样5 mL,分离血清和淋巴细胞,运用气相色谱-质谱(GC/MS)联用技术测定血清MTBE含量;运用彗星试验(comet assay),分析外周静脉血淋巴细胞DNA 损伤情况;运用Pearson 相关分析探索样本人群血清MTBE 含量与淋巴细胞DNA 损伤的相关性。

[结果] 接受体检的加油站调查对象未发现身体健康状况不合格者,均符合汽油从业人员的职业卫生要求;暴露组血清MTBE 含量平均值为(6.230& #177;2.369)μg/L,高于对照组[(5.164& #177;2.139)μg/L](P<0.05);暴露组Olive 尾矩平均值为(0.060& #177;0.045)μm,也高于对照组[(0.039& #177;0.038)μm](P<0.05);Pearson 相关分析显示,暴露组血清MTBE含量与淋巴细胞DNA损伤程度之间呈正相关关系(r=0.859,P<0.05)。

[结论] 血清MTBE含量与淋巴细胞DNA损伤可能存在相关性。
- 甲基叔丁基醚 /
- DNA损伤 /
- 淋巴细胞
Abstract:
[Objective] To probe the DNA damages induced by a gasoline additive, methyl tert-butyl ether (MTBE), in occupational exposed population.

[Methods] Physical examination and questionnaire survey were conducted in 100 workers selected from 8 gas stations in South China. A 5 mL peripheral venous blood sample was collected from each worker. Serum and lymphocytes were separated. The content of serum MTBE was detected with gas chromatography-mass spectrometry (GC/MS). Peripheral blood lymphocyte DNA damage was analyzed by comet assay. The relationship between serum MTBE content and lymphocyte DNA damage was explored using Pearson correlation analysis.

[Results] All selected workers met the basic health requirements for gasoline station operators. The mean value of MTBE in the exposure group was (6.230& #177;2.369) μg/L, significantly higher than that in the control group, (5.164& #177;2.139) μg/L (P< 0.05). The mean value of Olive tail moment in the exposure group was (0.060& #177;0.045) μm, significantly higher than that in the control group, (0.039& #177;0.038) μm (P< 0.05). The results of Pearson correlation analysis showed a positive correlation between MTBE content and the degree of DNA damage (r=0.859, P< 0.05).

[Conclusion] There is a correlation between serum MTBE content and lymphocyte DNA damage.
- methyl tert-butyl ether /
- DNA damage /
- lymphocytes