氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用

马萍, 陈姣娥, 甘亚平, 曾强, 毛琳, 杨旭

马萍, 陈姣娥, 甘亚平, 曾强, 毛琳, 杨旭. 氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用[J]. 环境与职业医学, 2013, 30(6): 465-470.
引用本文: 马萍, 陈姣娥, 甘亚平, 曾强, 毛琳, 杨旭. 氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用[J]. 环境与职业医学, 2013, 30(6): 465-470.
MA Ping , CHEN Jiao-e , GAN Ya-ping , ZENG Qiang , MAO Lin , YANG Xu . Oxidative Damages of Cypermethrin on Mouse Testis Tissues and Antioxidant Role of Vitamin E[J]. Journal of Environmental and Occupational Medicine, 2013, 30(6): 465-470.
Citation: MA Ping , CHEN Jiao-e , GAN Ya-ping , ZENG Qiang , MAO Lin , YANG Xu . Oxidative Damages of Cypermethrin on Mouse Testis Tissues and Antioxidant Role of Vitamin E[J]. Journal of Environmental and Occupational Medicine, 2013, 30(6): 465-470.

氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用

基金项目: 湖北省教育厅科学技术研究项目(编号:D20122803)
详细信息
    作者简介:

    马萍(1968-),女,硕士,副教授;研究方向:环境毒理学,E-mail:maping68@yahoo.com.cn

    通讯作者:

    杨旭,Email:yangxu@mail.ccnu.edu.cn

Oxidative Damages of Cypermethrin on Mouse Testis Tissues and Antioxidant Role of Vitamin E

More Information
    Corresponding author:

    YANG Xu ,Email:yangxu@mail.ccnu.edu.cn

  • 摘要:
    [目的] 研究氯氰菊酯(CP)对小鼠睾丸组织的氧化损伤和维生素E 的抗氧化作用。

    [方法] 以36 只昆明小鼠为受试动物, 随机分为6 组, 包括1 个阴性对照组、3 个氯氰菊酯染毒组、1 个维生素E 组和1 个高剂量氯氰菊酯(40 mg/kg)加维生素E组(100 mg/kg), 染毒组按10、20 和40 mg/kg 3 个剂量水平, 维生素E的剂量为100 mg/kg, 小鼠灌胃染毒7 d。取睾丸称重测定脏器系数; 以睾丸组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量; 以睾丸细胞测定DNA-蛋白质交联(DPC)系数; 并制作睾丸组织切片, 观察其病理损伤。

    [结果] 随着氯氰菊酯染毒剂量的升高, 睾丸的脏器系数、ROS、MDA含量和DPC系数逐渐上升, GSH含量逐渐降低, 各指标呈一定的剂量-效应关系。与对照组比较, 染毒剂量为20 mg/kg 时, 睾丸脏器系数(0.568& #177;0.027)、ROS 含量(586.2& #177;43.94)、GSH含量(18.15& #177;2.351)、DPC 系数(0.186& #177;0.017)和MDA含量(2.051& #177;0.137)差异有统计学意义(P < 0.05, P < 0.01), 睾丸部分生精小管生精细胞减少, 生精细胞脱落管腔; 染毒剂量为40 mg/kg 时, GSH含量(17.13& #177;1.203)、脏器系数(0.643& #177;0.028)、ROS 含量(898.8& #177;100.23)、MDA含量(2.299& #177;0.157)和DPC系数(0.229& #177;0.020)差异有统计学意义(P < 0.05, P < 0.01), 睾丸生精细胞明显减少, 部分生精细胞脱落坏死, 阻塞管腔。高剂量染毒加维生素E组与高剂量染毒组相比较, 睾丸的脏器系数、ROS、MDA含量和DPC 系数均有下降, GSH含量上升。ROS 含量(550.4& #177;102.3)、GSH含量(24.65& #177;2.892)和MDA含量(1.291& #177;0.289)差异有统计学意义(P < 0.05); 睾丸的脏器系数(0.507& #177;0.026)和DPC 系数(0.139& #177;0.017)差异有统计学意义(P < 0.01)。

    [结论] 较高剂量(20、40 mg/kg)的氯氰菊酯能造成小鼠睾丸的氧化损伤和病理损伤, 且可以被维生素E的抗氧化作用所拮抗。

     

    Abstract:
    [Objective] To investigate the oxidative damages of cypermethrin (CP) on mouse testis tissues and the antioxidant role of vitamin E.

    [Methods] Thirty-six Kuming mice were randomly divided into 6 groups and administered with designed agents by garage for 7 d. The groups included 1 negative control group, 3 CP groups (10, 20, and 40 mg/kg respectively), 1 protection group (40 mg/kg CP + 100 mg/kg vitamin E), and 1 vitamin E group (100 mg/kg). Afterwards, the mice were sacrificed and the testis organ coefficients were calculated. Reactive oxygen species (ROS), glutathione (GSH), and malondialdehyde (MDA) contents in the testis tissue homogenates were measured. Meanwhile, DNA-protein crosslink (DPC) coefficients were estimated from the testis cell suspension. The other testis tissues were prepared for sections and then stained for pathological observations under.

    [Results] The testis contents of ROS & MDA, the organ coefficients, and the DPC coefficients increased gradually in a dose-dependent manner, whereas the GSH contents decreased accordingly. Compared with the control group, the organ coefficient (0.568& #177;0.027), ROS content (586.2& #177;43.94), GSH content (18.15& #177;2.351), DPC coefficient (0.186& #177;0.017), and MDA content (2.051& #177;0.137) in the 20 mg/kg CP group were significantly elevated (P < 0.05 or P < 0.01). Besides, the tissue section of the mice in the 20 mg/kg CP group indicated a reduction and shedding of spermatogenic cells in the seminiferous tubules. In the 40 mg/kg CP group, the GSH content (17.13& #177;1.203), organ coefficient (0.643& #177;0.028), ROS contents (898.8& #177;100.23), the MDA content (2.299& #177;0.157), and DPC coefficient (0.229& #177;0.020) were significantly higher than those of the control group (P < 0.01 or P < 0.05). The spermatogenic cells reduced drastically and some fell off and blocked seminiferous tubules in the tissue section of the mice in the 40 mg/kg CP group. However, compared to the 40mg/kg CP group, the protection group showed significantly lower levels of organ coefficient (0.507& #177;0.026), ROS (550.4& #177;102.3), MDA (1.291& #177;0.289), and DPC (0.139& #177;0.017) (P < 0.05 or P < 0.01) levels, and higher GSH content (24.65& #177;2.892) (P < 0.05).

    [Conclusion] CP at relatively higher doses (20 mg/kg and 40 mg/kg) can induce oxidative stress and pathological damages in mouse testis tissues, while vitamin E can protect against the damages induced by CP.

     

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出版历程
  • 收稿日期:  2012-10-21
  • 刊出日期:  2017-06-23

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