Abstract:
[Objective] To investigate the oxidative damages of cypermethrin (CP) on mouse testis tissues and the antioxidant role of vitamin E.
[Methods] Thirty-six Kuming mice were randomly divided into 6 groups and administered with designed agents by garage for 7 d. The groups included 1 negative control group, 3 CP groups (10, 20, and 40 mg/kg respectively), 1 protection group (40 mg/kg CP + 100 mg/kg vitamin E), and 1 vitamin E group (100 mg/kg). Afterwards, the mice were sacrificed and the testis organ coefficients were calculated. Reactive oxygen species (ROS), glutathione (GSH), and malondialdehyde (MDA) contents in the testis tissue homogenates were measured. Meanwhile, DNA-protein crosslink (DPC) coefficients were estimated from the testis cell suspension. The other testis tissues were prepared for sections and then stained for pathological observations under.
[Results] The testis contents of ROS & MDA, the organ coefficients, and the DPC coefficients increased gradually in a dose-dependent manner, whereas the GSH contents decreased accordingly. Compared with the control group, the organ coefficient (0.568& #177;0.027), ROS content (586.2& #177;43.94), GSH content (18.15& #177;2.351), DPC coefficient (0.186& #177;0.017), and MDA content (2.051& #177;0.137) in the 20 mg/kg CP group were significantly elevated (P < 0.05 or P < 0.01). Besides, the tissue section of the mice in the 20 mg/kg CP group indicated a reduction and shedding of spermatogenic cells in the seminiferous tubules. In the 40 mg/kg CP group, the GSH content (17.13& #177;1.203), organ coefficient (0.643& #177;0.028), ROS contents (898.8& #177;100.23), the MDA content (2.299& #177;0.157), and DPC coefficient (0.229& #177;0.020) were significantly higher than those of the control group (P < 0.01 or P < 0.05). The spermatogenic cells reduced drastically and some fell off and blocked seminiferous tubules in the tissue section of the mice in the 40 mg/kg CP group. However, compared to the 40mg/kg CP group, the protection group showed significantly lower levels of organ coefficient (0.507& #177;0.026), ROS (550.4& #177;102.3), MDA (1.291& #177;0.289), and DPC (0.139& #177;0.017) (P < 0.05 or P < 0.01) levels, and higher GSH content (24.65& #177;2.892) (P < 0.05).
[Conclusion] CP at relatively higher doses (20 mg/kg and 40 mg/kg) can induce oxidative stress and pathological damages in mouse testis tissues, while vitamin E can protect against the damages induced by CP.