Abstract:
Objective To investigate the oxidative damages of cypermethrin (CP) on mouse testis tissues and the antioxidant role of vitamin E.
Methods Thirty-six Kuming mice were randomly divided into 6 groups and administered with designed agents by garage for 7 d. The groups included 1 negative control group, 3 CP groups (10, 20, and 40 mg/kg respectively), 1 protection group (40 mg/kg CP + 100 mg/kg vitamin E), and 1 vitamin E group (100 mg/kg). Afterwards, the mice were sacrificed and the testis organ coefficients were calculated. Reactive oxygen species (ROS), glutathione (GSH), and malondialdehyde (MDA) contents in the testis tissue homogenates were measured. Meanwhile, DNA-protein crosslink (DPC) coefficients were estimated from the testis cell suspension. The other testis tissues were prepared for sections and then stained for pathological observations under.
Results The testis contents of ROS & MDA, the organ coefficients, and the DPC coefficients increased gradually in a dose-dependent manner, whereas the GSH contents decreased accordingly. Compared with the control group, the organ coefficient (0.568& #177;0.027), ROS content (586.2& #177;43.94), GSH content (18.15& #177;2.351), DPC coefficient (0.186& #177;0.017), and MDA content (2.051& #177;0.137) in the 20 mg/kg CP group were significantly elevated (P < 0.05 or P < 0.01). Besides, the tissue section of the mice in the 20 mg/kg CP group indicated a reduction and shedding of spermatogenic cells in the seminiferous tubules. In the 40 mg/kg CP group, the GSH content (17.13& #177;1.203), organ coefficient (0.643& #177;0.028), ROS contents (898.8& #177;100.23), the MDA content (2.299& #177;0.157), and DPC coefficient (0.229& #177;0.020) were significantly higher than those of the control group (P < 0.01 or P < 0.05). The spermatogenic cells reduced drastically and some fell off and blocked seminiferous tubules in the tissue section of the mice in the 40 mg/kg CP group. However, compared to the 40mg/kg CP group, the protection group showed significantly lower levels of organ coefficient (0.507& #177;0.026), ROS (550.4& #177;102.3), MDA (1.291& #177;0.289), and DPC (0.139& #177;0.017) (P < 0.05 or P < 0.01) levels, and higher GSH content (24.65& #177;2.892) (P < 0.05).
Conclusion CP at relatively higher doses (20 mg/kg and 40 mg/kg) can induce oxidative stress and pathological damages in mouse testis tissues, while vitamin E can protect against the damages induced by CP.
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