运用TK基因突变试验及小鼠微核试验测定95%乙草胺原药的遗传毒性

Detection of Genetic Toxicity of 95% Acetochlor by TK Gene Mutation Test and Mice Micro-nucleus Test

摘要:
目的观察95%乙草胺原药的遗传毒性,为其安全使用及农药管理登记提供依据。

方法建立95%乙草胺原药的致突变试验模型,通过TK基因突变频率和微核率对其遗传毒性进行评价。

结果 95%乙草胺原药各剂量组TK6细胞TK位点突变频率与阴性对照组比较,差异均无统计学意义(P > 0.05);与阴性对照组比较, 95%乙草胺原药染毒组的骨髓嗜多染红细胞微核率未见明显增高(P > 0.05)。

结论在本试验条件下, 95%乙草胺原药无诱导小鼠骨髓细胞染色体损伤及致TK6细胞基因突变的作用。

Abstract:
Objective To observe the genetic toxicity of 95% acetochlor, in order to provide scientific basis for its safe use, registered pesticide management and control measures.

Methods Mutagenicity experimental models were set up. Genetic toxicity of 95% acetochlor was evaluated with TK gene mutation frequency and micronucleus rate.

Results No significant difference existed between every experimental group and negative control group for TK gene mutation frequency(P > 0.05). Compared with negative control group, the micronuleus rates of mice didn't show a statistical increase in every experimental group (P > 0.05).

Conclusion Under this experimental condition, 95% acetochlor cannot induce chromosome injury of mice polychromatic erythrocytes and gene mutation of TK6 cell.