Abstract:
[Objective] To study the role of cold stress played in the etiology of osteoarthritis in murine for build a cold stress-induced osteoarthritis model in mouse.
[Methods] Ten BALB/C mice a group in clean grade each were taken as model or control groups. The right knees of model group mice were stimulated with 6-8℃ water continuously for 30 days, 1.5 h/d. Then the degree of right knee swelling were measured with vernier caliper. The levels of TNF-α, rheumatoid factor(IgM) and hyaluronic acid (HA)in mice serum were detected with ELISA. The pathological slices of right knee were stained with hematoxylin-eosin(HE) dye for microscopy.
[Results] When the experiment ended(day 31), the diameters of right knee of mice in model group were longer than that of left knee(P<0.01). Compared with that of initial day, a significant difference of the diameter of right knees was also observed. However, there were no such significant differences in control group(P>0.01). Significant increases of serum TNF-α and HA levels were observed in the model group compared with the control group(P<0.01), while the level of IgM showed no significant difference(P>0.05). A series of pathological changes were found in model group, including disordered articular cartilage cells showed:karyolysis or disappearing cellular nucleus, remarkable proliferation, calcification, adhesion of cartilage or synovial cells, and so on.
[Conclusion] The cold stress could induce osteoarthritis in mice. The mice model of osteoarthritis was built preliminarily.
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