冷应激引发小鼠骨关节炎模型的建立

Study of Establishing the Cold Stress-Induced Osteoarthritis Model in Mouce

  • 摘要:
    目的 明确冷应激对小鼠膝骨关节炎发生的作用,为建立冷应激诱导的实验性小鼠骨关节炎模型提供参考。

    方法 选用清洁级BALB/C小鼠20 只,随机分为空白组和模型组,每组各10 只。给予模型组小鼠右膝关节6~8℃冷水刺激,30 d 后用游标卡尺测量关节肿胀程度,用酶联免疫吸附试验(ELISA法)测定小鼠血清肿瘤坏死因子(TNF-α)、类风湿因子(IgM)及透明质酸(HA)的含量,并取膝关节进行苏木精-伊红(HE)染色制作病理切片。

    结果 实验结束时(第31 天),模型组小鼠右膝关节直径大于左膝关节直径(P<0.01),且与第0 天时比较,右膝关节直径差异有统计学意义(P<0.01);而空白组小鼠左右膝关节直径及与第0 天时右膝关节直径相比,差异均无统计学意义(P>0.01)。与空白组相比,模型组血清TNF-α 和HA水平升高,差异有统计学意义(P<0.01);血清IgM的水平无变化(P>0.05)。模型组膝关节切片出现软骨及滑膜细胞排列紊乱,核溶或消失,有明显软骨及滑膜细胞增生、钙化、粘连及关节腔间隙变窄等骨关节炎病理改变。

    结论 冷水刺激可促进小鼠骨关节炎形成,小鼠骨关节炎建模初步成功。

     

    Abstract:
    Objective To study the role of cold stress played in the etiology of osteoarthritis in murine for build a cold stress-induced osteoarthritis model in mouse.

    Methods Ten BALB/C mice a group in clean grade each were taken as model or control groups. The right knees of model group mice were stimulated with 6-8℃ water continuously for 30 days, 1.5 h/d. Then the degree of right knee swelling were measured with vernier caliper. The levels of TNF-α, rheumatoid factor(IgM) and hyaluronic acid (HA)in mice serum were detected with ELISA. The pathological slices of right knee were stained with hematoxylin-eosin(HE) dye for microscopy.

    Results When the experiment ended(day 31), the diameters of right knee of mice in model group were longer than that of left knee(P<0.01). Compared with that of initial day, a significant difference of the diameter of right knees was also observed. However, there were no such significant differences in control group(P>0.01). Significant increases of serum TNF-α and HA levels were observed in the model group compared with the control group(P<0.01), while the level of IgM showed no significant difference(P>0.05). A series of pathological changes were found in model group, including disordered articular cartilage cells showed:karyolysis or disappearing cellular nucleus, remarkable proliferation, calcification, adhesion of cartilage or synovial cells, and so on.

    Conclusion The cold stress could induce osteoarthritis in mice. The mice model of osteoarthritis was built preliminarily.

     

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