石墨炉原子吸收光谱法测定全血中铍

Determination of Beryllium in Whole Blood with Graphite Furnace Atomic Absorbance Spectrometry

摘要:
目的建立石墨炉原子吸收光谱法测定全血中铍(Be)的方法。

方法全血样用 5%(v/v)硝酸脱去蛋白,经离心后取上清液进样, 以 0.5%(m/v)硝酸镁 Mg(NO₃)₂作为基体改进剂, 采用标准工作曲线法测定。

结果测定方法的线性范围为 0.18~5.00μg/L, 相关系数 (r)为 0.999 6, 相对标准偏差 (relative standard deviation, RSD)为 2.7%~4.2%,检出限为 0.27 μg/L, 加标回收率为 90%~100%。

结论该方法取样量少, 消解处理简单, 且快速、准确, 适用于全血中铍的测定。

Abstract:
Objective To establish the method for determination of beryllium in whole blood with graphite furnace atomic absorbance spectrometry.

Methods After the protein in whole blood was removed by nitric acid 5% (v/v) and centrifugal separation, it was determined by standard working curve using Mg (NO₃)₂ 0.5% (m/v) as matrix modifier.

Results The linearity range of the method was 0.18-5.00 μg/L, the correlation coefficient (r) was 0.999 6, the relative standard deviation (RSD) was 2.7%-4.2%, the detection limit was 0.27 μg/L, and the recovery was 90%-100%.

Conclusion This method is characterized by less amount of samples, simple digestion, better degree of precision and accuracy. It is fit to determine beryllium in whole blood.