大豆异黄酮对雄性子代大鼠<i>P450scc、MIS、ERβ</i>基因表达的影响

孙合涛; 纪海续; 周远忠; 范奇元

大豆异黄酮对雄性子代大鼠P450scc、MIS、ERβ基因表达的影响

Effects of Soy Isoflavone on Gene Expression of P450scc, MIS and ERβ in Male Offspring Rats

摘要

摘要:
目的探讨孕期大鼠暴露大豆异黄酮对子代雄性生殖系统发育的影响。

方法 SD大鼠按雌:雄=2:1的比例同笼交配获取40只孕鼠。将孕鼠随机分为5组:对照组(0 mg/kg);大豆异黄酮25、50、150、450 mg/kg组。在孕期第13~19天将大豆异黄酮以花生油为溶剂灌胃染毒。将雄性子代于出生后第18天处死取其睾丸组织用荧光定量PCR (Real-time PCR)检测胆固醇侧链裂解酶(P450scc)、苗勒管抑制物(MIS)和β-雌激素受体(ERβ)基因的表达水平。

结果大豆异黄酮可以明显降低ERβ基因的表达(P < 0.05);P450scc基因表达的降低不明显;在150 mg/kg和450 mg/kg剂量时可以增加MIS基因的表达(P < 0.01)。

结论孕期暴露大豆异黄酮后可以改变雄性子鼠睾丸的基因表达水平,从而对子鼠的雄性生殖系统发育造成影响。

Abstract:
Objective To explore the effects of soy isoflavone(SIF)on offspring male rats reproductive system after pregnancy exposure.

Methods 40 pregnant rats were divided into five groups randomly. Soy isoflavone was delivered to the pregnant rats by gavage (peanut oil worked as solution, was delivered to control group)during the period of E13~E19, with the doses of 0 (control group), 25, 50, 150, 450 mg/kg. On postnatal day 18, F1 male rats were sacrificed, testicular tissues were obtained, the expression of P450scc, MIS, ERβ mRNA in testis were measured by real-time PCR.

Results Compared with the control group, the expressions of ERβ (P < 0.01)and P450scc (P > 0.05)were deceased by exposed to different doses of SIF, but MIS expression increased (150, 450 mg/kg SIF, P < 0.01).

Conclusion SIF could alter reproductive-related gene expressions in the offspring male rat testes, which could lead to certain adverse effects.

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