Abstract:
[Objective] To find a rapid, precise, and high-throughput method for the determination of total urine mercury levels of occupational population by comparing several pretreatment methods of urine samples.
[Methods] Three pretreatment methods including microwave digestion, constant-temperature potassium permanganate oxidation, and acidic potassium bromatepotassium bromide (KBrO3/KBr) digestion system were applied under optimal conditions. Parameters of method performance such as accuracy, precision, sensitivity, recovery, and time for detection were compared.
[Results] The results of microwave digestion and KBrO3/KBr digestion system were in excellent agreement. The spiked recovery rates of the two methods were in the range of 101.5%-112.2% and 105.5%-110.0%, with relative standard deviations of 2.4% and 1.7%, respectively. The pretreatment time of the two methods was 6 h and 15 min, respectively. In addition, the results by constant-temperature potassium permanganate oxidation method were on the higher side with spiked recovery rate about 130%. Certified reference samples of lyophilized urine were used to validate the KBrO3/KBr digestion method and the results were in good agreement with the reference values.
[Conclusion] Acidic KBrO3/KBr digestion method is precise, efficient and rapid for the determination of total mercury in batches of urine samples of occupational population.
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