焦炉工人DNA损伤与组蛋白H2A单泛素化水平的关系

杨瑾; 李新; 郭庆峰; 王志武

焦炉工人DNA损伤与组蛋白H2A单泛素化水平的关系

Relationship between DNA Damage and Monoubiquitinated Histone H2A in Coke Oven Workers

摘要

摘要:
目的探讨焦炉工人DNA损伤与组蛋白H2A单泛素化水平的关系。

方法采用高效液相色谱法对焦化厂106 名焦炉工人(接触组)和73 名水处理厂工人(对照组)尿中1-羟基芘(1-hydroxypyrene, 1-OHP)浓度进行测定, 并采用彗星实验和蛋白质印迹法(Western blot)分别检测DNA损伤和组蛋白H2A单泛素化水平。

结果接触组Olive 尾矩(Olive tail moments, OTM)和尿1-OHP 的均值分别为0.46 和0.34 μmol/mol Cr, 均显著高于对照组(分别为0.37和0.21 μmol/mol Cr, P<0.05);而接触组单泛素化组蛋白H2A相对表达水平的中位数为0.61, 与对照组(0.91)相比为降低(P<0.05)。以OTM值为应变量, 多元线性回归显示自变量尿1-OHP 和单泛素化组蛋白H2A进入回归方程, 决定系数R²为0.383。

结论焦炉工人DNA损伤程度与尿1-OHP 含量和单泛素化组蛋白H2A相对表达水平关系密切。

Abstract:
Objective To examine the relationship between DNA damage and monoubiquitinated histone H2A in coke oven workers.

Methods A total of 106 coke oven workers(exposed group)and 73 water treatment workers(control group)were recruited in this study. The levels of urinary 1-hydroxypyrene(1-OHP)were determined using high performance liquid chromatography. Comet assay and Western blot were used to detect the DNA damage and the monoubiquitylation of histone H2A, respectively.

Results The mean Olive tail moments(OTM)(0.46)and urinary 1-OHP concentrations(0.34 μmol/mol Cr)in the exposed group were significantly higher than those in the control group(0.37 and 0.21 μmol/mol Cr, respectively, P<0.05). But the median of expression of monoubiquitinated histone H2A(0.61)in the exposed group were significantly lower than that in the control group(0.91, P<0.05). By taking OTM as the dependent variable, the results of multiple linear regression analysis showed that urinary 1-OHP and the relative expressions of monoubiquitinated histone H2A fit the equation as independent variables, and the coefficient of determination R² was 0.383.

Conclusion DNA damage and urinary 1-OHP are closely related with the relative expression of monoubiquitinated histone H2A in the coke oven workers.

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