Abstract:
Objective To investigate the cytotoxicity and DNA damage in Chinese hamster lung fibroblast cells (V79 cells) induced by N, N-dimethylformamide (DMF) in vitro.
Methods Tetrazolium (MTT) assay and single cell gel electrophoresis were used to detect the cytotoxicity and DNA damage in V79 cells after exposure to 0.5, 2.0, 8.0, 32.0, and 128.0 mmol/L of DMF for 6 h, 12 h, and 24 h.
Results DMF significantly reduced the viability of cultured V79 cells with the same exposure time periods (P<0.05) and in a dose-dependent manner (6 h, b=-0.002, P<0.05; 12 h, b=-0.003, P<0.05; 24 h, b=-0.003, P<0.05). There were significant differences in the comet tails rate, tail length, Olive tail moment, and tail DNA percentage between different exposure groups and the control group for the same exposure time periods (P<0.05).
Conclusion Under current experimental condition, DMF can obviously inhibit the proliferation of V79 cells, showing a positive dose-dependent relationship, and can cause DNA damage.
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