苯并(a)芘致人支气管上皮细胞DNA损伤及与组蛋白H2A单泛素化水平的关系

王志武, 贾利娜, 牛侨, 杨瑾

王志武, 贾利娜, 牛侨, 杨瑾. 苯并(a)芘致人支气管上皮细胞DNA损伤及与组蛋白H2A单泛素化水平的关系[J]. 环境与职业医学, 2012, 29(3): 136-138,143.
引用本文: 王志武, 贾利娜, 牛侨, 杨瑾. 苯并(a)芘致人支气管上皮细胞DNA损伤及与组蛋白H2A单泛素化水平的关系[J]. 环境与职业医学, 2012, 29(3): 136-138,143.
WANG Zhi-wu , JIA Li-na , NIU Qiao , YANG Jin . Correlation between DNA Damage and Expression of Mono-ubiquitinated Histone H2A in 16HBE Cells Exposed to Benzo[a]pyrene[J]. Journal of Environmental and Occupational Medicine, 2012, 29(3): 136-138,143.
Citation: WANG Zhi-wu , JIA Li-na , NIU Qiao , YANG Jin . Correlation between DNA Damage and Expression of Mono-ubiquitinated Histone H2A in 16HBE Cells Exposed to Benzo[a]pyrene[J]. Journal of Environmental and Occupational Medicine, 2012, 29(3): 136-138,143.

苯并(a)芘致人支气管上皮细胞DNA损伤及与组蛋白H2A单泛素化水平的关系

基金项目: 国家自然科学基金项目(编号:30901180)
详细信息
    作者简介:

    王志武(1986-),男,硕士生;研究方向:分子毒理学,E-mail:muwangzhiwu@163.com

    通讯作者:

    牛侨,Email:niuqiao55@163.com

    杨瑾,Email:yj750410@163.com

Correlation between DNA Damage and Expression of Mono-ubiquitinated Histone H2A in 16HBE Cells Exposed to Benzo[a]pyrene

More Information
    Corresponding author:

    NIU Qiao ,Email:niuqiao55@163.com

    YANG Jin ,Email:yj750410@163.com

  • 摘要:
    [目的] 通过体外细胞培养途径,探讨苯并(a)芘(benzo[a]pyrene,B[a]P)作用下人支气管上皮细胞(16HBE)DNA损伤与组蛋白H2A单泛素化表达水平的关系。

    [方法] 取对数生长期16HBE细胞,采用2 μmol/L B[a]P染毒不同时间(0、1、2、4、8、12、24、48 h)进行实验,用碱性单细胞凝胶电泳实验检测细胞DNA损伤水平,并以Olive尾矩值(olivetail moments,OTM)评价DNA损伤程度;用Western-blot法检测组蛋白H2A单泛素化水平。

    [结果] 单细胞凝胶电泳结果显示:2μmol/L B[a]P染毒不同时间时,随着染毒时间的延长,各染毒组细胞DNA损伤程度均明显增高,与对照组相比,差异均有统计学意义(P<0.01);Western-blot结果显示:2 μmol/L B[a]P染毒不同时间时,与对照组相比,组蛋白H2A的单泛素化水平增高,且在染毒2 h后,差异有统计学意义(P<0.01);回归分析显示,单泛素化H2A的表达水平与DNA损伤程度存在高度相关,决定系数(R2)为0.910,P<0.01。

    [结论] B[a]P染毒致16HBE细胞DNA损伤与组蛋白H2A单泛素化水平之间存在密切关系。

     

    Abstract:
    [Objective] To investigate the association between DNA damage and expression of mono-ubiquitinated histone H2A in human bronchial epithelial cells (16HBE) exposed to benzo[a]pyrene (B[a]P).

    [Methods] The 16HBE cells collected at the logarithmic phase of growth were exposed to B[a]P at the concentration of 2μmol/L for 0, 1, 2, 4, 8, 12, 24 and 48 h respectively. DNA damage was determined by comet assay and expressed as olive tail moments (OTM). Protein expression was detected by Western-blot.

    [Results] The results of comet assay showed that the OTM values increased with exposure time and a significant in crement was found as compared with the control group (P<0.01). After exposed to 2μmol/L B[a]P for 2 h, the mono-ubiquitination levels of histone H2A increased significantly when compared with the control group (P<0.01). The regression analysis showed a significant correlation between OTM and the levels of mono-ubiquitinated histone H2A, and the determination coefficient was 0.910 (P<0.01).

    [Conclusion] The findings suggest a close correlation between DNA damage and the levels of mono-ubiquitinated histone H2A in 16HBE cells exposed to B[a]P.

     

计量
  • 文章访问数:  2433
  • HTML全文浏览量:  8
  • PDF下载量:  657
  • 被引次数: 0
出版历程
  • 收稿日期:  2011-07-09
  • 刊出日期:  2017-06-23

目录

    /

    返回文章
    返回