Abstract:
Objective To investigate cytotoxicity, DNA damage and reactive oxygen species (ROS) of fibroblasts of L929 cell line induced by indium sulfate (IS) in vitro.
Methods The L929 cells cultured in vitro were exposed to IS at varied dosages (1, 2, 4 and 8 mmol/L) for different exposure periods (2, 24 and 48 h). The cytotoxicity were observed by methyl thiazolyl tetrazolium (MTT) colorimetric assay. Single cell gel electrophoresis (SCGE) was used to detect the DNA damage and ROS concentration of L929 cells after exposed to different concentrations (1, 2 and 4 mmol/L) of IS for 2 h.
Results The absorbency of the interferred cells turned lower than that of the control group along with the IS concentration increasing. The difference was significant between the exposed cells and the controls (P<0.05). SCGE results showed that the comet cells ratio, the tail DNA%, the tail length, the Olive tail moment and the concentration of ROS increased with the increasing IS concentrations. The statistical differences among the control group and the various exposed groups were significant (P<0.05).
Conclusion Under current experimental conditions, IS presents cytotoxicity on L929 cells in dose-and time-dependent manner, and it can also induce DNA single-strand break (SSB) and increase ROS in a relatively short period of time.
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