煤烘高氟玉米染毒大鼠的血清TRACP-5b表达变化

韦艳; 张震; 刘新儒; 张华

煤烘高氟玉米染毒大鼠的血清TRACP-5b表达变化

Expression Variation of Serum TRACP-5b in Rats Treated with Coal-Burning Fluorine

摘要

摘要:
目的观察煤烘高氟玉米染毒大鼠的血清抗酒石酸酸性磷酸酶5b(TRACP-5b)的表达变化,探讨骨吸收在氟骨症发生机制中的作用。

方法将断乳4 周的SD大鼠120 只随机分为4 组,对照组给予商品化饲料,3 个实验组在对照饲料基础上给予不同比例煤烘高氟玉米,低、中、高剂量染毒组饲料含氟量分别为9.56、15.89 及23.00 mg/kg。于连续染毒至第30、90、180 天时,检测大鼠骨氟、尿氟含量及氟斑牙发生率、股骨病理组织学积分,ELISA法检测血清TRACP-5b 水平。

结果 (1)各剂量组骨氟、尿氟、氟斑牙发生率及股骨病理学积分均随着染毒剂量的增加而升高(P< 0.05)。染毒至第30、90、180 天时,各剂量组的骨氟、尿氟均高于对照组(P< 0.05),且剂量较高的组别的尿氟、骨氟高于剂量较低的组别(P< 0.05)。染毒至第90 天和180 天时,各剂量组氟斑牙发生率高于对照组(P< 0.05);中、高剂量组股骨病理切片积分高于低剂量组和对照组(P< 0.05)。(2)各组染毒至180 天时,血清TRACP-5b 均高于同剂量组90 天及30 天时的水平(P< 0.05)。在第30 天及90 天时,各组血清TRACP-5b 差异无统计学意义;染毒至180 天时,高剂量组TRACP-5b 高于对照组和中剂量组(P< 0.05),其余各组间差异无统计学意义。

结论摄入煤烘高氟玉米剂量较高,且摄入时间较长时,可能引起血清TRACP-5b 表达升高。

Abstract:
Objective To observe the expression variation of serum tartrate-resistant acid phosphatase isoform 5b (TRACP-5b) in rats exposed to coal-burning fluorine, and explore the role of bone resorption in the pathogenesis of skeletal fluorosis.

Methods One hundred and twenty SD rats weaned for 4 weeks were divided into 4 groups: 1 control group was fed with commercial feed, and the other 3 experimental groups were fed with mixed feeds which contained 9.56 mg/kg (low-dose group), 15.89 mg/kg (mediumdose group), and 23.00 mg/kg (high-dose group) of fluorine. The animals were executed after 30 d, 90 d, and 180 d of treatment to determine bone and urinary fluoride content, incidence rate of dental fluorosis, and femur pathological score. TRACP-5b levels in serum were tested by ELISA.

Results (1) The bone and urinary fluoride concentrations, the dental fluorosis incidences, and the femur pathological scores of the 3 experimental groups were significantly increased in a dose-dependent manner respectively (all P< 0.05). After the treatment for 30 d, 60 d, and 90 d, the urinary and bone fluoride concentrations of each experimental group were statistically higher than the control group (all P< 0.05), and those of the higher dose group were remarkably higher than those of the lower dose group respectively (all P< 0.05). For 90 d and 180 d, the incidence of dental fluorosis in each dose group was obviously higher than that of controls (all P< 0.05); the femur pathological score of the medium-dose and the high-dose groups were significantly higher than that of the low-dose group and the controls (all P< 0.05). (2) The serum TRACP-5b levels in each experimental group with 180 d of exposure were statistically higher than those in the same dose group with 30 d and 90 d of exposure (all P< 0.05). There was no significant difference in the serum TRACP-5b among the groups after treatment for 30 d and 90 d. After the treatment for 180 d, the TRACP-5b levels of the high-dose group was significantly higher than that of the controls (all P< 0.05), and no significantly differences were found among the other groups.

Conclusion Exposure to high doses of fluorine for a longterm basis may cause elevated expression of serum TRACP-5b.

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