活性氧在p,p'-DDE诱导大鼠睾丸支持细胞凋亡中的作用

宋杨; 石玉琴; 程晋; 关霞; 王玉萍; 杨克敌

活性氧在p,p'-DDE诱导大鼠睾丸支持细胞凋亡中的作用

基金项目: 国家自然科学基金资助项目(编号:30671734)

详细信息

作者简介:
宋杨(1982-),女,博士生,研究方向:环境毒理学研究

通讯作者:
杨克敌,Email:yangkd@mails.tjmu.edu.cn

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出版历程
- 收稿日期: 2008-11-28
- 刊出日期: 2017-06-25

The Role of Reactive Oxygen Species in p, p'-DDE-induced Apoptosis of Sertoli Cells in Rats

More Information

Corresponding author:
YANG Ke-di ,Email:yangkd@mails.tjmu.edu.cn

摘要

摘要:
[目的] 研究活性氧在2,2-双-(对氯苯基)-1,1-二氯乙稀(p,p'-DDE)诱导大鼠睾丸支持细胞凋亡中的作用。

[方法] 从大鼠睾丸组织中分离支持细胞进行离体原代培养3d,用0、10、30、50μmol/L的p,p'-DDE及加有300μmol/L的抗氧化剂N-乙酰-L-半胱氨酸(NAC)的p,p'-DDE (50μmol/L)继续培养24h,应用流式细胞仪测定细胞内活性氧(ROS)水平、线粒体膜势能(△ψm)、凋亡发生率。

[结果] 50μmol/L的p,p'-DDE可以诱导支持细胞ROS显著升高,10、30、50μmol/L p,p'-DDE处理组的线粒体膜势能下降,30、50μmol/L的p,p'-DDE可以诱导支持细胞凋亡,与对照组相比,差异有统计学意义(P<0.05);NAC有效抑制了ROS升高,削弱线粒体膜势能下降,减少了凋亡发生率,与50μmol/L的p,p'-DDE处理组相比,差异有显著意义(P<0.05)。

[结论] p,p'-DDE可以诱导大鼠睾丸支持细胞凋亡,ROS产生可能是其重要原因之一。
- 2,2-双-对氯苯基-1,1-二氯乙稀 /
- 活性氧 /
- 睾丸支持细胞 /
- 凋亡
Abstract:
[Objective] To investigate the mechanism of p,p'-DDE-induced apoptosis of Sertoli cells and the role of reactive oxygen species.

[Methods] Sertoli cells from testes of rats were treated with 0, 10, 30, and 50 μmol/L p,p'-DDE, or with 50 μmol/L p,p'-DDE pretreated by 300 μmolFL NAC (N-acetyl-L-cysteine). Then the fluoresence intensity of reactive oxygen species(ROS), mitochondria membrane potential(△ψm)and apoptosis percentage was detected by a FACS Calibur flow cytometer.

[Results] 50 μmol/L p,p'-DDE increased the ROS level. The mitochondria membrane potential was decreased after all p,p'-DDE's treatments. The percentage of apoptosis was increased after treatment with 30 or 50 μmol/L p,p'-DDE. Compared with the control group, the difference was statistically significant(P<0.05). The antioxidant NAC inhibited the increased ROS generation, decreased the mitochondria membrane potential and apoptosis percentage (P<0.05).

[Conclusion] p,p'-DDE could induce apoptosis in Sertoli cells. The generation of ROS may be an important reason in the apoptosis.
- p,p'-DDE /
- ROS /
- Sertoli cells /
- apoptosis