Abstract:
Objective To explore the protective effect of grape seed oil (GSO) on DNA oxidative damage of liver cells in rats exposed to CdCl2.
Methods A total of 40 SD rats of SPF grade were randomly divided into 5 groups, which were the control group (intraperitoneal injection and gastric perfusion of normal saline with same volume), the CdCl2 exposure-positive group (intraperitoneal injection of 1.0mg/kg CdCl2 and gastric perfusion of normal saline with the same volume), and the GSO low-, medium-and high-dose intervention groups (intraperitoneal injection of 1.0 mg/kg CdCl2 and gastric perfusion of 0.66 mg/kg, 1.32 mg/kg, 2.64 mg/kg GSO, respectively) with 8 of either gender in each group. Malondialdehyde (MDA) content in liver was measured by thiobarbituric acid (TBA) method. DNA oxidative damage was measured by single cell gel eletrophoresis (SCGE).
Results The 1.0 mg/kg CdCl2 induced a significant increase in MDA content and DNA oxidative damage. The difference of MDA contents between the GSO high-dose intervention group and the CdCl2 exposure-positive group was significant (P<0.05). The clearances in the GSO low -, medium-and high-dose intervention groups were 54.55%, 54.55% and 100.00%, respectively. The DNA damage significantly decreased in the GSO intervention groups in a dose-dependent manner, compared with the CdCl2 exposure-positive group (P<0.05).
Conclusion A certain dose of grape seed oil can induce a decrease in MDA content and DNA damage in rat liver after cadmium exposure.
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