Abstract:
[Objective] To analyze the toxic effects of organic pollutants extracted from pipeline water in G City on the reproductive organ weights and hormone levels of male rats.
[Methods] Organic pollutants in pipeline water samples from a district of G City were extracted by solid phase extraction method. Forty SD adult male rats were divided into solvent control group(administered with corn oil), low dose group(administered with organic pollutants extraction, OPE, 3 L/kg body weight per day), moderate dose group(OPE, 15 L/kg body weight per day), and high dose group(OPE, 75 L/kg body weight per day). OPE were administered to the rats once a day, and continuously for 28 days. The rats were sacrificed after OPE administration ended and serum samples were collected for luteinizing hormone(LH), testosterone(T), and estradiol(E2)measurement by chemiluminescence.
[Results] Compared with the control group, no obvious changes were observed in the weights of testis and epididymis in the 3 exposed groups(P>0.05). With the increase of OPE dosage, the level of rats, serum LH decreased, but compared with the solvent control group, no statistical significance existed(P>0.05). The level of E2 obviously increased in a dose-dependent manner, especially in the moderate and the high dose groups(P<0.05 or P<0.01). The levels of T in the 3 exposed groups were ascending at first and followed by descending along with the increment of OPE dosage, but a significant decline was only observed in the high dose group(P<0.05).
[Conclusion] Exposure to moderate and high doses of OPE extracted from the pipeline water in G city can induce damages to testis leydig cells, destroy the balance of reproductive hormones, and finally result in reproductive dysfunction.
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