Abstract:
Objective To study the genotoxicity of extractable organic matter (EOM) in vegetable greenhouse soil.
Methods EOM obtained by soxhlet extraction from greenhouse soil were fed to 3 groups of mice via gavage at concentrations of 5, 15, and 30 g soil dry weight/kg body weight, once per day for 4 weeks. A negative control group was administered with dimethyl sulfoxide and a positive control group with cyclophosphamide. Chromosomal damage in peripheral blood lymphocytes and DNA injury in peripheral blood cells were detected by cytokinesis-block micronucleus (CBMN) assay and comet assay independently.
Results There were significant differences in the micronucleus frequency between the EOM exposure groups and the negative control group, and the micronucleus frequency remarkably increased with EOM concentration. Compared with the negative control group, the tail length, tail DNA percent, and Olive tail moment increased in the middle and the high dose groups.
Conclusion EOM extracted from greenhouse soil could induce chromosomal damage and DNA injury in mice.
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