Abstract:
Objective To explore the effect of paraquet (PQ) on the gene expression of critical molecules, β-catenin and DVL2, in Wnt signaling pathway during differentiation process of neural stem cells.
Methods Using the ReNcell CX cell model, the differentiation induced in vivo was observed. After treatment with various concentrations of PQ, cell viability was measured by tetrazolium (MTT) assay. After the cells exposed with non-cytotoxic concentrations of 0.00, 0.10, 1.00, and 10.00 μmol/L PQ, real-time reverse transcription-polymerase chain reaction was adopted to detect mRNA expressions of β-catenin and DVL2 on 2, 4, 8, and 12 d of differentiation.
Results The cell viability was significantly inhibited after administered with 100.00 μmol/L of PQ (P < 0.01). PQ significantly down-regulated β-catenin mRNA expressions on 2, 8, and 12 d of differentiation (P < 0.01) though no obvious changes were observed between the 10.00 μmol/L group and the control group on 8 d of differentiation. In addition, PQ significantly down-regulated DVL2 mRNA expressions on 2 d and 12 d of differentiation compared to the control group (P < 0.01) though DVL2 mRNA expression increased at 0.10 μmol/L after 12 d of differentiation. But the DVL2 mRNA expressions significantly increased on 4 d of differentiation (P < 0.01).
Conclusion PQ can suppress key factors β-catenin and DVL2 in Wnt signaling, which could affect differentiation of human neural stem cells.
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