红景天多糖对长波紫外线致大鼠氧化损伤的保护作用

黄冰洋; 魏海; 周权明; 郭婧; 钟丽婷; 沈楠; 姜艳霞

红景天多糖对长波紫外线致大鼠氧化损伤的保护作用

Protective Effect of Rhodiola Rosea Polysaccharide on UVA-induced Oxidative Damage in Rats

摘要

摘要:
目的探讨红景天多糖对长波紫外线(ultraviolet A, UVA)辐照大鼠损伤的防护效果。

方法用红景天多糖干预UVA辐射大鼠,检测各组血清、肝脏匀浆中超氧化物歧化物 (superoxide dismutase, SOD)、谷胱甘肽 (glutathione,GSH), 丙二醛(malondialdehyde, MDA)及羟自由基, 观察红景天多糖的防护效果。

结果 981.71 J/cm² UVA辐射组血清中 SOD、GSH、抑制羟自由基能力明显低于正常对照组(P < 0.05), MDA含量明显高于正常对照组(P < 0.05)。经高剂量红景天多糖干预的 UVA辐射组血清中 GSH和抑制羟自由基能力明显高于 UVA辐射组(P < 0.05), MDA含量明显低于UVA 辐射组(P < 0.05); 低剂量组 MDA 含量明显低于 UVA 辐射组(P < 0.05), 抑制羟自由基能力明显高于 UVA 辐射组(P < 0.05)。肝组织匀浆中, UVA辐射组 SOD活性、抑制羟自由基能力明显低于正常对照组(P < 0.05); 高剂量组 SOD活性、抑制羟自由基能力明显高于 UVA辐射组(P < 0.05), MDA含量明显低于 UVA辐射组(P < 0.05)。

结论红景天多糖对 UVA辐照所导致的氧化损伤有一定的修复能力, 具体机制尚有待进一步研究。

Abstract:
Objective To explore the protective effect of rhodiola rosea polysaccharide on the ultraviolet A (UVA) radiated rats.

Methods The UVA radiated rats were interfered with rhodiola rosea polysaccharides and detected for superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA) and hydroxyl radical in serum and liver homogenate to observe the role of rhodiola rosea polysaccharide in protection.

Results The serum SOD, GSH and inhibition ability of hydroxyl radical in 981.71 J/cm² UVA group were significantly lower than that in control group (P < 0.05), but MDA in that test group was higher than that in control group (P < 0.05). In the high dose rhodiola rosea polysaccharide group, serum GSH and inhibition ability of hydroxyl radical were significantly higher than that in UVA radiation group (P < 0.05), and MDA was significantly slower than that in UVA radiation group (P < 0.05); while in the low dose rhodiola rosea polysaccharide group, MDA was obviously lower (P < 0.05) and inhibition ability of hydroxyl radical was significantly higher (P < 0.05) than that in UVA radiation group. In the liver homogenate, SOD activity and hydroxyl radical inhibition ability in UVA radiation group were significantly lower than that in control group (P < 0.05), and that in high dose rhodiola rosea polysaccharide group were significantly higher than that in UVA radiation group (P < 0.05), but MDA was obviously lower (P < 0.05).

Conclusion Rhodiola rosea polysaccharide has definite ability to repair UVA radiation damage, but its mechanism need to be further studied.

HTML全文

参考文献(0)

施引文献

补充资料

审稿意见

勘误/撤稿