Abstract:
Objective To understand the distribution of genotypes and mutation of hepatitis B virus (HBV) in hepatitis B patients.
Methods Eighty-eight hepatitis B patients in Shanghai were randomly sampled in 2010. Nested polymerase chain reaction (PCR) was carried out to detect genotypes of HBV in blood samples of the selected patients. The pre-S/S and C gene regions of HBV DNA were amplified and sequenced.
Results Among the 88 subjects, 52 patients (59.09%) were infected by HBV genotype C and 36 patients (40.91%) by HBV genotype B. The mean age of HBV genotype B group (38.56& #177;12.55) years old was lower than that of HBV genotype C group (44.54& #177;15.72) years old with significantly statistical difference (P<0.05). The positive rate of hepatitis B e antigen (HBeAg) in the HBV genotype B group (66.67%) was significantly higher than that of the HBV genotype C group (28.85%) (P<0.05). The mutation rates of C1762 and C1764 situs in HBV genotype C group were 50% and 50.77% respectively, statistically higher than those of the genotype B group which were 27.78% and 25% respectively (P<0.05).
Conclusion The hepatitis B patients in this study are dominantly infected with genotype C. The mutation rates of C1762 and C1764 situs in patients with genotype C were higher than those with genotype B. Strengthened surveillance on genotype and mutation of HBV is required.
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