慢性复合应激对大鼠胰岛β细胞功能的影响

孙静; 李伟; 张俊权; 李秀平; 王生

慢性复合应激对大鼠胰岛β细胞功能的影响

Effects of Chronic Multiple Stress on Pancreas β Cell Function in Rats

摘要

摘要:
目的研究慢性复合应激对大鼠胰岛β细胞的影响。

方法将40只SD大鼠随机分成应激组和对照组,每组20只。应激组实施8周的慢性复合应激刺激,对照组在此期间正常饲养。检测两组大鼠血糖、胰岛素,口服糖耐量实验(OGTT),计算胰岛素分泌指数(HOMA-β)、OGTT曲线下面积、早期相胰岛素分泌指数(ΔI₃₀/ΔG₃₀)、β细胞功能修正指数(MBCI),并检测胰岛β细胞凋亡情况。

结果慢性复合应激8周后,与对照组相比,应激组大鼠空腹血糖明显升高(P < 0.001),空腹胰岛素水平和HOMA-β明显降低(分别为P < 0.05,P < 0.01);同时与对照组相比,应激组大鼠葡萄糖曲线下面积明显增加(P < 0.05)、胰岛素曲线下面积、ΔI₃₀/ΔG₃₀以及MBCI明显减少(分别为P < 0.05,P < 0.05,P < 0.01)。细胞凋亡结果显示应激组大鼠凋亡率明显高于对照组(P < 0.001)。

结论慢性复合应激使大鼠胰岛β细胞功能降低。

Abstract:
Objective To explore the effect of chronic multiple stress on pancreas β cell function in rats.

Methods Forty male Sprague-Dawley rats were randomly divided into stress and control groups with twenty in each. Plasma glucose, insulin, insulin resistance, β cell function, and oral glucose tolerance test(OGTT)were measured and calculated. β cell apoptosis were detected using Tunel measurement.

Results Compared with the control group, the stress group had significantly higher fasting plasma glucose (FPG)and increased area under the curves of blood glucose levels (AUC_G) (P < 0.001, P < 0.05). The fasting insulin(FINS), homeostasis model assessment(HOMA)-β, the areas under the curves of serum insulin levels(AUC_I), ΔI₃₀/ΔG₃₀ and modified β cell index (MBCI)were significantly lower (P < 0.05, P < 0.01, P < 0.05, P < 0.05, P < 0.01)in the stress group than the control group. The apoptosis rate of the stress group was significantly higher than the control group (P < 0.001).

Conclusion It is observed that chronic multiple stress reduced the pancreas βcell function in rats.

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