Abstract:
Objective To observe the changes of intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) in Chinese hamster V79 cells induced by indium sulfate in vitro.
Methods The cultured V79 cells were exposed to indium sulfate at various dosages (0.5, 1.0, 2.0, 4.0 and 8.0 mmol/L). The cell survival rate was checked with methyl thiazolyl tetrazolium (MTT). The changes of intracellular ROS and Δψm in V79 cells were analyzed by flow cytometry with 2', 7'-dichlorodihydrofluorescin diacetate (DCFH-DA) and 5, 5', 6, 6'-tetrachloro -1, 1', 3, 3'-tetraethyl-imidacarbocyanine iodide (JC-1) probe after being interfered for 12 h.
Results Indium sulfate significantly reduced the survival rate of V79 cells. After exposed to indium sulfate at various dosages for 12 h, the mean intracellular fluorescence intensity obviously increased from 21.60 to 31.10 while the Δψm significantly decreased from 0.191 3 to 0.109 0. The statistical differences were significant between the control group and the exposure groups (P<0.05).
Conclusion Indium sulfate can increase the release of intracellular ROS and reduce the Δψm significantly. The damage of indium sulfate to V79 cells might be associated with the change of normal cellular metabolism.
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